English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/49866
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Identification and analysis of conserved cis-regulatory regions of the MEIS1 gene

AuthorsRoyo, José Luis ; Bessa, José ; Hidalgo, Carmen; Fernández-Miñán, Ana ; Tena, Juan J. ; Roncero, Yolanda; Gómez-Skarmeta, José Luis ; Casares, Fernando
Issue Date20-Mar-2012
PublisherPublic Library of Science
CitationPLoS ONE 7(3): e33617 (2012)
AbstractMeis1, a conserved transcription factor of the TALE-homeodomain class, is expressed in a wide variety of tissues during development. Its complex expression pattern is likely to be controlled by an equally complex regulatory landscape. Here we have scanned the Meis1 locus for regulatory elements and found 13 non-coding regions, highly conserved between humans and teleost fishes, that have enhancer activity in stable transgenic zebrafish lines. All these regions are syntenic in most vertebrates. The composite expression of all these enhancer elements recapitulate most of Meis1 expression during early embryogenesis, indicating they comprise a basic set of regulatory elements of the Meis1 gene. Using bioinformatic tools, we identify a number of potential binding sites for transcription factors that are compatible with the regulation of these enhancers. Specifically, HHc2:066650, which is expressed in the developing retina and optic tectum, harbors several predicted Pax6 sites. Biochemical, functional and transgenic assays indicate that pax6 genes directly regulate HHc2:066650 activity.
DescriptionThis is an open-access article distributed under the terms of the Creative Commons Attribution License.
Publisher version (URL)http://dx.doi.org/10.1371/journal.pone.0033617
URIhttp://hdl.handle.net/10261/49866
DOI10.1371/journal.pone.0033617
Identifiersdoi: 10.1371/journal.pone.0033617
e-issn: 1932-6203
Appears in Collections:(CABD) Artículos
Files in This Item:
File Description SizeFormat 
MEIS1.pdf1,36 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.