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CE-TOF MS analysis of complex protein hydrolyzates from genetically modified soybeans – A tool for foodomics

AuthorsSimó, Carolina ; Marina, María Luisa ; Cifuentes, Alejandro
Shotgun proteomics
Transgenic foods
Issue DateApr-2010
CitationElectrophoresis 31(7): 1175-1183 (2010)
AbstractA CE-TOF MS proteomic approach was applied for the analysis of hydrolyzates from complex soybean protein mixtures. After CE-TOF MS method development, the new approach provided the simultaneous analysis of more than 150 peptides from the soybean protein fraction soluble in ACN-water (80/20 v/v). The method is fast (about 30 min of analysis per sample) and is characterized by a relatively low running cost. The approach was used to study the substantial equivalence between a genetically modified variety of soybean compared with its traditional counterpart. No significant differences were found between the two studied soybeans based on the protein fraction studied. The capacity of the CE-TOF MS method to analyze complex mixtures of peptides in short times opens interesting possibilities in the growing Foodomics area.
Description9 páginas, 7 figuras.-- El pdf del artículo es la versión de autor.-- et al.
Publisher version (URL)http://dx.doi.org/10.1002/elps.200900448
Appears in Collections:(IFI) Artículos
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