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Título

The use of green fluorescent protein as a marker for Brucella vaccines

AutorChacón Diaz, Carlos; Muñoz-Rodríguez, Melissa; Barquero-Calvo, Elías; Guzmán Verri, Caterina; Chaves Olarte, Esteban; Grilló, María Jesús ; Moreno Robles, Eduardo
Palabras claveBrucella
Brucellosis
Vaccines
S19
Rev1
GFP
Diagnostic tests
ELISA
Fecha de publicación10-ene-2011
EditorElsevier
CitaciónVaccine 29(3): 577-582 (2011)
ResumenBrucellosis is an important malady of productive and wildlife animals and a worldwide zoonosis. The use of effective vaccines and the corresponding diagnostic tests that allow differentiating infected from vaccinated animals are essential tools to control the disease. For this, a prototype of Brucella abortus S19 vaccine expressing green fluorescent protein (S19-GFP) was constructed. The S19-GFP was readily identified under ultraviolet light by macroscopic and microscopic examination and maintained all the biochemical characteristics of the parental S19 vaccine. S19-GFP replicated ex vivo and in vivo, and protected mice against challenge with virulent B. abortus to the same extent as the isogenic S19. An immunoenzymatic assay designed to measure anti-GFP antibodies allowed the discrimination between mice vaccinated with S19-GFP and those immunized with S19. Both vaccines raised antibodies against lipopolysaccharide molecule to similar levels. This experimental model constitutes a “proof of concept” for the use of Brucella-GFP vaccines and associated diagnostic tests to distinguish vaccinated from naturally Brucella infected animals.
Descripción6 p., 3 figures, 1 table and references
Versión del editorhttp://dx.doi.org/10.1016/j.vaccine.2010.09.109
URIhttp://hdl.handle.net/10261/49692
DOI10.1016/j.vaccine.2010.09.109
ISSN0264-410X
E-ISSN1873-2518
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