English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/49645
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Título

Evidence for a non-replicative intracellular stage of nontypable Haemophilus influenzae in epithelial cells

AutorMorey, Pau; Cano, Victoria; Martí-Lliteras, Pau; López-Gómez, Antonio; Regueiro, Verónica; Saus, Carles; Bengoechea, José Antonio ; Garmendia, Juncal
Fecha de publicaciónene-2011
EditorSociety for General Microbiology
CitaciónMicrobiology 157(1): 234-250 (2011)
ResumenNontypable Haemophilus influenzae (NTHi) is a Gram-negative, non-capsulated human bacterial pathogen, a major cause of a repertoire of respiratory infections, and intimately associated with persistent lung bacterial colonization in patients suffering from chronic obstructive pulmonary disease (COPD). Despite its medical relevance, relatively little is known about its mechanisms of pathogenicity. In this study, we found that NTHi invades the airway epithelium by a distinct mechanism, requiring microtubule assembly, lipid rafts integrity, and activation of phosphatidylinositol 3-kinase (PI3K) signalling. We found that the majority of intracellular bacteria are located inside an acidic subcellular compartment, in a metabolically active and non-proliferative state. This NTHi-containing vacuole (NTHi-CV) is endowed with late endosome features, co-localizing with LysoTracker, lamp-1, lamp-2, CD63 and Rab7. The NTHi-CV does not acquire Golgi- or autophagy-related markers. These observations were extended to immortalized and primary human airway epithelial cells. By using NTHi clinical isolates expressing different amounts of phosphocholine (PCho), a major modification of NTHi lipooligosaccharide, on their surfaces, and an isogenic lic1BC mutant strain lacking PCho, we showed that PCho is not responsible for NTHi intracellular location. In sum, this study indicates that NTHi can survive inside airway epithelial cells.
Descripción17 p., 6 figures and references
Versión del editorhttp://dx.doi.org/10.1099/mic.0.040451-0
URIhttp://hdl.handle.net/10261/49645
DOI10.1099/mic.0.040451-0
ISSN1350-0872
E-ISSN1465-2080
Aparece en las colecciones: (IDAB) Artículos
(DPUB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
restringido.pdf21,67 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.