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Dietary fat modulates calorie restriction-induced changes of apoptotic markers in mouse skeletal muscle

AuthorsLópez-Domínguez, José A.; López-Lluch, Guillermo ; Fernández del Río, Lucía; Navas, Plácido ; Ramsey, Jon J.; Burón, María I.; Villalba, José M.
Calorie restriction (CR)
Skeletal muscle
Metabolic activity
Issue Date12-Dec-2011
PublisherSociedad Española de Biología Celular
CitationXIV Congresos de la Sociedad Española de Biología Celular (2011)
AbstractAgeing is a progressive and irreversible process leading to a general loss of function. In skeletal muscle, a postmitotic tissue exhibiting a low regenerative rate, several factors which include increased levels of apoptosis, result in a decline in mass and function, known as sarcopenia. Calorie restriction without malnutrition (CR) has been proved to prevent or delay several ageassociated pathophysiological changes in rodents. Although the mechanisms underlying this effect have to be fully determined, both a decrease in oxidative damage and a change in membranes composition appear to be involved. In our work we studied the synergic effect of six-month 40% calorie restriction and variations in fat composition of diets. We established three groups of C57BL/6 mice subjected to CR with diets containing lard, soybean oil or fish oil as their primary fat source respectively. To determine whether any of the diets exhibits a preventive role on skeletal muscle apoptosis, some markers of apoptotic cell death were studied, namely caspases 3, 8 and 9, Bax levels, and plasma membrane-bound neutral sphingomyelinase activity. Additionally, levels of sarcolemmal coenzyme Q9 and Q10 and lipid hydroperoxides were measured for a better characterization of the cellular redox status. Decreased levels of sphingomyelinase activity, lipid hydroperoxides and Bax were observed as a response to calorie restriction. Additionally, a fish oil-based diet resulted in general and significant changes such as additional decreases in caspase 8 and 9 activities, or increases in sarcolemmal coenzyme Q9 and Q10 content. CR regulated parameters mentioned above were positively influenced by this diet with the only exception of caspase 3 activity, for which no significant changes were observed. Further studies involving long-term calorie restriction and diet variations in old mice will soon help to better establish the extent to which apoptotic processes are regulated by membrane composition or membrane-linked metabolic activity.
DescriptionResumen del póster presentado al XIV Congresos de la Sociedad Española de Biología Celular, celebrado en Málaga del 12 al 15 de diciembre de 2011.
Appears in Collections:(CABD) Comunicaciones congresos
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