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Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/49634
Título

Wine yeast molecular typing using a simplified method for simultaneously extracting mtDNA, nuclear DNA and virus dsRNA

AutorMaqueda, Matilde; Zamora, Emiliano; Rodríguez-Cousiño, Nieves; Ramírez, Manuel
Palabras claveYeast typing
Nucleic acid extraction
mtDNA
Nuclear DNA
dsRNA viruses
Fecha de publicaciónabr-2010
EditorElsevier
CitaciónFood Microbiology 27(2): 205-209 (2010)
ResumenQuick and accurate methods are required for the identification of industrial, environmental, and clinical yeast strains. We propose a rapid method for the simultaneous extraction of yeast mtDNA, nuclear DNA, and virus dsRNA. It is simpler, cheaper, and faster than the previously reported methods. It allows one to choose among a broad range of molecular analysis approaches for yeast typing, avoiding the need to use of several different methods for the separate extraction of each nucleic acid type. The application of this method followed by the combined analysis of mtDNA and dsRNA (ScV-M and W) is a highly attractive option for fast and efficient wine yeast typing.
Descripción5 páginas, 4 figuras, 1 tabla.
Versión del editorhttp://dx.doi.org/10.1016/j.fm.2009.10.004
URIhttp://hdl.handle.net/10261/49634
DOI10.1016/j.fm.2009.10.004
ISSN0740-0020
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