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Title

Characterization of progenitor cells with high vascular endothelial engraftment potential during mouse development

AuthorsCañete, Ana ; Prados, Isabel ; Sánchez, María José
KeywordsProgenitor cells
Embryonic cells
Vascular
Hematopoietic stem cells (HSCs)
Issue Date12-Dec-2011
PublisherSociedad Española de Biología Celular
CitationXIV Congresos de la Sociedad Española de Biología Celular (2011)
AbstractCells that can contribute to vasculature in vivo have exciting clinical potential for the regeneration of damaged vessels and ex vivo re-construction of organs. Few cell types have been identified that can integrate/differentiate to vascular cells in non-ischemic tissue upon transplantation. Among these are the blood-related cells derived from the adult bone marrow. However, they present limited vascular endothelial differentiation capability once transplanted. We inquired if embryonic/fetal cells from hematopoietic locations presented incremented vascular-endothelial repopulation potential upon transplantation. Using the hemato-vascular reporter vector SCL-3’Enh for hemato-vascular progenitor isolation and endothelial lineage tracing, our group showed that transplanted SCL3’Enh-PLAPbright+ fetal liver cells derived from E12 mouse embryos possessed incremented long-term hematopoietic and liver vascularendothelial repopulation activity when compared to adult bone marrow cells (Garcia Ortega, 2010; Silberstein, 2005). Donor derived endothelial cells were classified into microvascular-endothelium clusters and scattered endothelium associated to middle/big vessels. To address the relation of vascular and hematopoietic repopulation potential during embryo development we performed a spatial/temporal mapping. We transplanted cells from E12 embryos obtained from locations containing hematopoietic stem cells (HSCs): AGM, yolk sac, fetal liver and placenta. Extensive microvascular repopulation activity was only observed in AGM and FL hematopoietic chimeras. However, donor derived scattered endothelium was detected in all hematopoietic chimeras, independently of the embryonic origin of the donor cells. Moreover, cells with microvascular repopulation potential appear in the fetal liver at day 11 of development, before the emergence of HSCs, and expands up to day 14. Also, all E11-AGM derived hematopoietic chimeras presented scattered endothelium, but no microvascular patches. This data indicates that scattered endothelium and hematopoietic repopulation activity emerge together whereas the progenitor cell population with microvascular repopulation activity is not derived from HSCs. Further data will be presented on FACS phenotypic characterization of this new fetal endothelial progenitor population.
DescriptionResumen del póster presentado al XIV Congresos de la Sociedad Española de Biología Celular, celebrado en Málaga del 12 al 15 de diciembre de 2011.
URIhttp://hdl.handle.net/10261/49630
Appears in Collections:(CABD) Comunicaciones congresos
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