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Título

Immune responses of mussel hemocyte subpopulations are differentially regulated by enzymes of the PI 3-K, PKC, and ERK kinase families

AutorGarcía-García, Erik; Prado-Álvarez, María CSIC ORCID; Novoa, Beatriz CSIC ORCID; Figueras Huerta, Antonio ; Rosales, Carlos CSIC
Palabras claveMollusc
Signal transduction
Innate immunity
Phagocytosis
Respiratoty burst
Wortmannin
Staurosporine
PD98059
Professional phagocytes
Fecha de publicación2008
EditorElsevier
CitaciónDevelopmental and Comparative Immunology 32(6): 637-653 (2008)
ResumenVarious hemocyte cell types have been described in invertebrates, but for most species a functional characterization of different hemocyte cell types is still lacking. In order to characterize some immunological properties of mussel (Mytilus galloprovincialis) hemocytes, cells were separated by flow cytometry and their capacity for phagocytosis, production of reactive oxygen species (ROS), and production of nitric oxide (NO), was examined. Phosphatidylinositol 3-kinase (PI 3-K), protein kinase C (PKC), and extracellular signal-regulated kinase (ERK) inhibitors were also used to biochemically characterize these cell responses. Four morphologically distinct subpopulations, designated R1–R4, were detected. R1, R2, and R3 cells presented different levels of phagocytosis towards zymosan, latex beads, and two bacteria species. Similarly, R1 to R3, but not R4, cells produced ROS, while all subpopulations produced NO, in response to zymosan. Internalization of all phagocytic targets was blocked by PI 3-K inhibition. In addition, internalization of latex particles, but not of bacteria, was partially blocked by PKC or ERK inhibition. Interestingly, phagocytosis of zymosan was impaired by PKC, or ERK inhibitors, only in R2 cells. Zymosan-induced ROS production was blocked by PI 3-K inhibition, but not by PKC, or ERK inhibition. In addition, zymosan-stimulated NO production was affected by PI 3-K inhibition in R1 and R2, but not in R3 or R4 cells. NO production in all cell types was unaffected by PKC inhibition, but ERK inhibition blocked it in R2 cells. These data reveal the existence of profound functional and biochemical differences in musselhemocytes and indicate that M. galloprovincialishemocytes are specialized cells fulfilling specific tasks in the context of host defense.
Descripción17 páginas, 11 figuras
Versión del editorhttp://dx.doi.org/10.1016/j.dci.2007.10.004
URIhttp://hdl.handle.net/10261/49623
DOI10.1016/j.dci.2007.10.004
ISSN0145-305X
E-ISSN1879-0089
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