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dc.contributor.authorCotobal, Cristina-
dc.contributor.authorSegurado, Mónica-
dc.contributor.authorAntequera, Francisco-
dc.identifier.citationEMBO Journal 29(5): 934-942 (2010)es_ES
dc.descriptionEl pdf del artículo es la versión pre-print.es_ES
dc.description.abstractDNA replication origins (ORI) in Schizosaccharomyces pombe colocalize with adenine and thymine (A+T)-rich regions, and earlier analyses have established a size from 0.5 to over 3 kb for a DNA fragment to drive replication in plasmid assays. We have asked what are the requirements for ORI function in the chromosomal context. By designing artificial ORIs, we have found that A+T-rich fragments as short as 100 bp without homology to S. pombe DNA are able to initiate replication in the genome. On the other hand, functional dissection of endogenous ORIs has revealed that some of them span a few kilobases and include several modules that may be as short as 25–30 contiguous A+Ts capable of initiating replication from ectopic chromosome positions. The search for elements with these characteristics across the genome has uncovered an earlier unnoticed class of low-efficiency ORIs that fire late during S phase. These results indicate that ORI specification and dynamics varies widely in S. pombe, ranging from very short elements to large regions reminiscent of replication initiation zones in mammals.es_ES
dc.description.sponsorshipCC was supported by a postgraduate fellowship from the Spanish Ministerio de Ciencia e Innovación (MICINN) and this work was funded by grants BFU2008-01919BMC and Consolider-Ingenio CSD2007-00015 (from MICINN).es_ES
dc.publisherNature Publishing Groupes_ES
dc.subjectCell cyclees_ES
dc.subjectDNA replicationes_ES
dc.subjectGenome organizationes_ES
dc.subjectReplication dynamicses_ES
dc.subjectReplication origines_ES
dc.titleStructural diversity and dynamics of genomic replication origins in Schizosaccharomyces pombees_ES
dc.description.peerreviewedPeer reviewedes_ES
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