English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/49619
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Structural diversity and dynamics of genomic replication origins in Schizosaccharomyces pombe

AuthorsCotobal, Cristina ; Segurado, Mónica ; Antequera, Francisco
KeywordsCell cycle
DNA replication
Genome organization
Replication dynamics
Replication origin
Issue Date2010
PublisherNature Publishing Group
CitationEMBO Journal 29(5): 934-942 (2010)
AbstractDNA replication origins (ORI) in Schizosaccharomyces pombe colocalize with adenine and thymine (A+T)-rich regions, and earlier analyses have established a size from 0.5 to over 3 kb for a DNA fragment to drive replication in plasmid assays. We have asked what are the requirements for ORI function in the chromosomal context. By designing artificial ORIs, we have found that A+T-rich fragments as short as 100 bp without homology to S. pombe DNA are able to initiate replication in the genome. On the other hand, functional dissection of endogenous ORIs has revealed that some of them span a few kilobases and include several modules that may be as short as 25–30 contiguous A+Ts capable of initiating replication from ectopic chromosome positions. The search for elements with these characteristics across the genome has uncovered an earlier unnoticed class of low-efficiency ORIs that fire late during S phase. These results indicate that ORI specification and dynamics varies widely in S. pombe, ranging from very short elements to large regions reminiscent of replication initiation zones in mammals.
DescriptionEl pdf del artículo es la versión pre-print.
Publisher version (URL)http://dx.doi.org/10.1038/emboj.2009.411
URIhttp://hdl.handle.net/10261/49619
DOI10.1038/emboj.2009.411
ISSN0261-4189
E-ISSN1460-2075
Appears in Collections:(IMB) Artículos
(IBFG) Artículos
Files in This Item:
File Description SizeFormat 
Structural diversity and dynamics.pdf313,87 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.