English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/49568
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

A laboratory guide to in vitro fertilization of oceanic squids

AuthorsVillanueva, Roger ; Bozzano, Anna ; Petroni, Giuliano ; Quintana, D.
KeywordsCephalopoda
Teuthida
Gametes
Embryonic development
Paralarvae
Issue Date15-Apr-2012
PublisherElsevier
CitationAquaculture 342–343: 125–133 (2012)
AbstractIn vitro fertilization of oceanic squid is a necessary step to develop their larval culture and creates new opportunities to study and understand cephalopod development, taxonomy and ecology. The techniques described here in the form of a laboratory guide represent an attempt to refine and standardize the general methodology by indicating suitable laboratory materials, sources and preservation of gametes, and methods for fertilization and egg incubation. Twelve oceanic squid species have been fertilized in vitro to date; we outline a generalized experimental protocol and suggest that the reader consider particular species-specific modifications. Inadequate egg chorion expansion and premature hatching are identified as major challenges for in vitro fertilization. Recommendations for future research include studies on optimal gamete concentration, gamete preservation and determination of the functions of female oviducal and nidamental glands. The greatest obstacles to improving fertilization success in squids are the lack of standard methodologies and the paucity of information on both endogenous and exogenous factors controlling the fertilization process. This review is a first step toward overcoming these challenges
DescriptionVillanueva, Roger ... et al. 9 pages, 2 figures, 1 table
Publisher version (URL)http://dx.doi.org/10.1016/j.aquaculture.2012.02.025
URI10261/49568
DOI10.1016/j.aquaculture.2012.02.025
ISSN0044-8486
Appears in Collections:(ICM) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.