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dc.contributor.author | Alasaad, Samer | - |
dc.contributor.author | Soriguer, Ramón C. | - |
dc.contributor.author | Abu-Madi, Marawan | - |
dc.contributor.author | El Behairy, Ahmed | - |
dc.contributor.author | Díez Baños, Pablo | - |
dc.contributor.author | Píriz, Ana | - |
dc.contributor.author | Fickel, Joerns | - |
dc.contributor.author | Sánchez, Antonio | - |
dc.date.accessioned | 2012-05-10T09:49:45Z | - |
dc.date.available | 2012-05-10T09:49:45Z | - |
dc.date.issued | 2011-06 | - |
dc.identifier.citation | Parasitol Res (2011) 108:1513–1517 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10261/49508 | - |
dc.description.abstract | The present study aimed to establish a fluorescence- based polymerase chain reaction-linked single-strand conformation polymorphism (F-PCR-SSCP) assay for the identification of Fasciola spp. Based on the sequences of the second internal transcribed spacer (ITS-2) of the nuclear ribosomal DNA, we designed a set of genus- specific primers for the amplification of Fasciola ITS-2, with an estimated size of 140 bp. These primers were labelled by fluorescence dyes, and the PCR products were analyzed by capillary electrophoresis under non- denaturing conditions (F-PCR-SSCP). Capillary electropho- resis analysis of the fluorescence-labelled DNA fragments displayed three different peak profiles that allowed the accurate identification of Fasciola species: one single peak specific for either Fasciola hepatica or Fasciola gigantica and a doublet peak corresponding to the “intermediate” Fasciola. Validation of our novel method was performed using Fasciola specimens from different host animals from China, Spain, Nigeria, and Egypt. This F-PCR-SSCP assay provides a rapid, simple, and robust tool for the identification and differentiation between Fasciola spp. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Springer Nature | es_ES |
dc.rights | openAccess | es_ES |
dc.title | A fluorescence-based polymerase chain reaction-linked single-strand conformation polymorphism (F-PCR-SSCP) assay for the identification of Fasciola spp | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1007/s00436-010-2209-z | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1007/s00436-010-2209-z | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.languageiso639-1 | en | - |
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