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dc.contributor.authorAlasaad, Samer-
dc.contributor.authorSoriguer, Ramón C.-
dc.contributor.authorAbu-Madi, Marawan-
dc.contributor.authorEl Behairy, Ahmed-
dc.contributor.authorDíez Baños, Pablo-
dc.contributor.authorPíriz, Ana-
dc.contributor.authorFickel, Joerns-
dc.contributor.authorSánchez, Antonio-
dc.identifier.citationParasitol Res (2011) 108:1513–1517es_ES
dc.description.abstractThe present study aimed to establish a fluorescence- based polymerase chain reaction-linked single-strand conformation polymorphism (F-PCR-SSCP) assay for the identification of Fasciola spp. Based on the sequences of the second internal transcribed spacer (ITS-2) of the nuclear ribosomal DNA, we designed a set of genus- specific primers for the amplification of Fasciola ITS-2, with an estimated size of 140 bp. These primers were labelled by fluorescence dyes, and the PCR products were analyzed by capillary electrophoresis under non- denaturing conditions (F-PCR-SSCP). Capillary electropho- resis analysis of the fluorescence-labelled DNA fragments displayed three different peak profiles that allowed the accurate identification of Fasciola species: one single peak specific for either Fasciola hepatica or Fasciola gigantica and a doublet peak corresponding to the “intermediate” Fasciola. Validation of our novel method was performed using Fasciola specimens from different host animals from China, Spain, Nigeria, and Egypt. This F-PCR-SSCP assay provides a rapid, simple, and robust tool for the identification and differentiation between Fasciola spp.es_ES
dc.titleA fluorescence-based polymerase chain reaction-linked single-strand conformation polymorphism (F-PCR-SSCP) assay for the identification of Fasciola sppes_ES
dc.description.peerreviewedPeer reviewedes_ES
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