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Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/49485

The THP1-SAC3-SUS1-CDC31 Complex Works in Transcription Elongation-mRNA Export Preventing RNA-mediated Genome Instability

AutorGonzález-Aguilera, Cristina ; Tous, Cristina ; Gómez-González, Belén ; Huertas Sánchez, Pablo ; Luna, Rosa ; Aguilera, Andrés
Palabras claveCDC31 protein
Calcium-Binding proteins
Cell cycle proteins
Nuclear proteins
Nucleocytoplasmic transport proteins
Activation induced cytidine deaminase (AICDA)
Cytidine deaminase
Fecha de publicación1-oct-2008
EditorAmerican Society for Cell Biology
CitaciónMolecular Biology of the Cell 19(10): 4310-4318 (2008)
ResumenThe eukaryotic THO/TREX complex, involved in mRNP biogenesis, plays a key role in the maintenance of genome integrity in yeast. mRNA export factors such as Thp1-Sac3 also affect genome integrity, but their mutations have other phenotypes different from those of THO/TREX. Sus1 is a novel component of SAGA transcription factor that also associates with Thp1-Sac3, but little is known about its effect on genome instability and transcription. Here we show that Thp1, Sac3, and Sus1 form a functional unit with a role in mRNP biogenesis and maintenance of genome integrity that is independent of SAGA. Importantly, the effects of ribozyme-containing transcription units, RNase H, and the action of human activation-induced cytidine deaminase on transcription and genome instability are consistent with the possibility that R-loops are formed in Thp1-Sac3-Sus1-Cdc31 as in THO mutants. Our data reveal that Thp1-Sac3-Sus1-Cdc31, together with THO/TREX, define a specific pathway connecting transcription elongation with export via an RNA-dependent dynamic process that provides a feedback mechanism for the control of transcription and the preservation of genetic integrity of transcribed DNA regions.
Descripción9 páginas, 7 figuras. Monitoring editor: Marvin P. Wickens.
Versión del editorhttp://dx.doi.org/10.1091/mbc.E08-04-0355
ReferenciasPMID: 18667528
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