English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/49437
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Nab2 functions in the metabolism of RNA driven by polymerases II and III

AuthorsGonzález-Aguilera, Cristina ; Tous, Cristina ; Babiano, Reyes; Cruz, Jesús de la; Luna, Rosa ; Aguilera, Andrés
KeywordsNab2 protein
Nucleocytoplasmic transport proteins
RNA
Saccharomyces cerevisiae proteins
Poly A
RNA polymerase II
RNA polymerase III
Cell nucleus
Issue Date1-Aug-2011
PublisherAmerican Society for Cell Biology
CitationMolecular Biology of the Cell 22(15): 2729-2740 (2011)
AbstractGene expression in eukaryotes is an essential process that includes transcription, RNA processing, and export. One important player in this interface is the poly(A)+-RNA–binding protein Nab2, which regulates the mRNA poly(A)+-tail length and export. Here we show that Nab2 has additional roles during mRNA transcription, tRNA metabolism, and ribosomal subunit export. Nab2 is associated with the entire open reading frame of actively transcribed RNA polymerase (RNAP) II and III genes. As a consequence, nab2 mutations confer translation defects that are detected by polysome profiling. Genome-wide analysis of expression of a conditional degron nab2 mutant shows that the role of Nab2 in RNAPII transcription and RNAPIII metabolism is direct. Taken together, our results identify novel functions for Nab2 in transcription and metabolism of most types of RNAs, indicating that Nab2 function is more ubiquitous than previously anticipated, and that it is a central player in the general and coordinated control of gene expression from transcription to translation.
DescriptionThis article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
Publisher version (URL)http://dx.doi.org/10.1091/mbc.E11-01-0055
URIhttp://hdl.handle.net/10261/49437
DOI10.1091/mbc.E11-01-0055
ISSN1059-1524
E-ISSN1939-4586
Appears in Collections:(CABIMER) Artículos
Files in This Item:
File Description SizeFormat 
Nab2_2011.pdf2,39 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.