English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/49410
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Identification and diversity of multiresistant Corynebacterium striatum clinical isolates by MALDI-TOF mass spectrometry and by a multigene sequencing approach

AutorGomila, Margarita; Renom, Feliu; Gallegos, Maria del Carmen; Garau, Margarita; Guerrero, Dolores; Soriano, Joan B.; Lalucat, Jorge
Fecha de publicación4-abr-2012
EditorBioMed Central
CitaciónBMC Microbiology. 04;12(1):52 (2012)
ResumenAbstract Background The genus Corynebacterium is composed of Gram-positive bacteria that are widely distributed throughout the environment; these bacteria are also part of the normal microbiota of human skin and mucous membranes. Multiple studies have shown that species of this genus, including C. striatum, become pathogenic to humans under special conditions. Our aim was to determine the characteristics of clinical multiresistant strains of C. striatum that were isolated in our geographical region, to determine their diversity, and to compare them with the type strain and with related species. We studied fifty-two strains of C. striatum isolated from different hospitals from Mallorca, Spain, mainly from the Hospital Joan March in Bunyola, Mallorca. Most of the strains were isolated from sputum cultures of respiratory samples from patients with chronic obstructive pulmonary disease. To gain further insight into the genetic diversity of the strains, we analysed several housekeeping genes and other genes associated with antibiotic resistance. Strains were also characterised phenotypically by their antibiotic resistance profiles and by MALDI-TOF mass spectrometry analysis. Results The ITS1 region, gyrA and rpoB were chosen as the appropriate genes in the C. striatum genome to study the genetic diversity of C. striatum species and to discriminate between strains. After analysing these three genes, four sequence types (ST2, ST4, ST1 and ST11) were found to be the most abundant. Splits tree analysis of the strains demonstrated that these clinical isolates did not share any alleles with the type strain of the species. Recombination was detected within all of the C. striatum isolates, and different clonal populations were detected within the samples. Conclusions Our results demonstrate that the isolates were best identified using gene-based molecular methods; using these methods, the isolated strains were determined to be different from the type strain of C. striatum. The ITS1 region and the gyrA and rpoB genes were selected because of their variability and were the most useful tools for discriminating between strains. The phenotype and antibiotype characteristics of the strains did not seem suitable for typing purposes. MALDI-TOF mass spectrometry can be a useful method for identifying and discriminating between C. striatum strains.
Versión del editorhttp://dx.doi.org/10.1186/1471-2180-12-52
Aparece en las colecciones: (IMEDEA) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
1471-2180-12-52.xml94,92 kBXMLVisualizar/Abrir
1471-2180-12-52-S6.DOC289,5 kBMicrosoft WordVisualizar/Abrir
1471-2180-12-52-S5.DOC262 kBMicrosoft WordVisualizar/Abrir
1471-2180-12-52-S1.DOC114,5 kBMicrosoft WordVisualizar/Abrir
1471-2180-12-52.pdf537,88 kBAdobe PDFVista previa
1471-2180-12-52-S4.DOC98,5 kBMicrosoft WordVisualizar/Abrir
1471-2180-12-52-S2.DOCX15,61 kBMicrosoft Word XMLVisualizar/Abrir
1471-2180-12-52-S3.DOC169 kBMicrosoft WordVisualizar/Abrir
Mostrar el registro completo

Artículos relacionados:

NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.