English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/49407
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Título

Transfer of R388 derivatives by a pathogenesis-associated type IV secretion system into both bacteria and human cells

AutorFernández-González, Esther ; De Paz, Héctor D. ; Alperi, Anabel ; Agúndez, Leticia ; Sangari, Félix J. ; Llosa, Matxalen
Fecha de publicación2011
EditorAmerican Society for Microbiology
CitaciónJournal of Bacteriology 193(22): 6257-6265 (2011)
ResumenBacterial type IV secretion systems (T4SSs) are involved in processes such as bacterial conjugation and protein translocation to animal cells. In this work, we have switched the substrates of T4SSs involved in pathogenicity for DNA transfer. Plasmids containing part of the conjugative machinery of plasmid R388 were transferred by the T4SS of human facultative intracellular pathogen Bartonella henselae to both recipient bacteria and human vascular endothelial cells. About 2% of the human cells expressed a green fluorescent protein (GFP) gene from the plasmid. Plasmids of different sizes were transferred with similar efficiencies. B. henselae codes for two T4SSs: VirB/VirD4 and Trw. A ΔvirB mutant strain was transfer deficient, while a ΔtrwE mutant was only slightly impaired in DNA transfer. DNA transfer was in all cases dependent on protein TrwC of R388, the conjugative relaxase, implying that it occurs by a conjugation-like mechanism. A DNA helicase-deficient mutant of TrwC could not promote DNA transfer. In the absence of TrwB, the coupling protein of R388, DNA transfer efficiency dropped 1 log. The same low efficiency was obtained with a TrwB point mutation in the region involved in interaction with the T4SS. TrwB interacted with VirB10 in a bacterial two-hybrid assay, suggesting that it may act as the recruiter of the R388 substrate for the VirB/VirD4 T4SS. A TrwB ATPase mutant behaved as dominant negative, dropping DNA transfer efficiency to almost null levels. B. henselae bacteria recovered from infected human cells could transfer the mobilizable plasmid into recipient Escherichia coli under certain conditions, underscoring the versatility of T4SSs.
DescripciónTrabajo presentado al: "Workshops Current Trends in Biomedicine" organizado por la Universidad Internacional de Andalucía y celebrado en Baeza (España) del 24 al 26 de octubre de 2011.
Versión del editorhttp://dx.doi.org/10.1128/​JB.05905-11
URIhttp://hdl.handle.net/10261/49407
DOI10.1128/​JB.05905-11
ISSN0021-9193
Aparece en las colecciones: (IBBTEC) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Transfer of R388.pdf729,52 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.