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| Título: | Two distinct calcium pools in the endoplasmic reticulum of HEK-293T cells |
Autor: | Aulestia, Francisco J.; Rodríguez-García, Arancha CSIC; Alonso, María Teresa; García-Sancho, Javier CSIC ORCID | Palabras clave: | Aequorin Calcium microdomains Endoplasmic reticulum Intracellular calcium store |
Fecha de publicación: | 2011 | Editor: | Biochemical Society | Citación: | Biochemical Journal 435: 227-235 (2011) | Resumen: | Agonist-sensitive intracellular Ca2+ stores may be heterogeneous and exhibit distinct functional features. We have studied the properties of intracellular Ca2+ stores using targeted aequorins for selective measurements in different subcellular compartments. Both, HEK-293T [HEK (human embryonic kidney)-293 cells expressing the large T-antigen of SV40 (simian virus 40)] and HeLa cells accumulated Ca2+ into the ER (endoplasmic reticulum) to near millimolar concentrations and the IP3-generating agonists, carbachol and ATP, mobilized this Ca2+ pool. We find in HEK-293T, but not in HeLa cells, a distinct agonist-releasable Ca2+ pool insensitive to the SERCA (sarco/endoplasmic reticulum Ca2+ ATPase) inhibitor TBH [2,5-di-(t-butyl)-benzohydroquinone]. TG (thapsigargin) and CPA (cyclopiazonic acid) completely emptied this pool, whereas lysosomal disruption or manoeuvres collapsing endomembrane pH gradients did not. Our results indicate that SERCA3d is important for filling the TBH-resistant store as: (i) SERCA3d is more abundant in HEK-293T than in HeLa cells; (ii) the SERCA 3 ATPase activity of HEK-293T cells is not fully blocked by TBH; and (iii) the expression of SERCA3d in HeLa cells generated a TBH-resistant agonist-mobilizable compartment in the ER. Therefore the distribution of SERCA isoforms may originate the heterogeneity of the ER Ca2+ stores and this may be the basis for store specialization in diverse functions. This adds to recent evidence indicating that SERCA3 isoforms may subserve important physiological and pathophysiological mechanisms. | Descripción: | 10 páginas, 7 figuras.-- et al.-- El pdf del artículo es la versión post-print. | Versión del editor: | http://dx.doi.org/10.1042/BJ20101427 | URI: | http://hdl.handle.net/10261/49405 | DOI: | 10.1042/BJ20101427 | ISSN: | 0264-6021 | E-ISSN: | 1470-8728 |
| Aparece en las colecciones: | (IBGM) Artículos |
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| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| Two distinct calcium pools.pdf | 477,43 kB | Adobe PDF |  Visualizar/Abrir |
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