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An Enhanced Liquid Chromatographic Method For 5-Hydroxymethylfurfural Determination in UHT Milk

AuthorsMorales, F. J. ; Romero, C.; Jiménez Pérez, Salvio
KeywordsColumn liquid chromatography
Heat-treated milk
Milk quality indicators
Issue DateJan-1992
CitationChromatographia 33(1/2): 45-48 (1992)
AbstractAn enhanced method of HPLC for the determination of 5-Hydroxymethyl-2-furfuraldehyde (BMF) in fluid milk wasperfected. Its resolving capacity permitted the specificseparation of this compound. The real concentration(IJmol/l) of HMF was determined and not the HMF value, as in the colorimetric analysis, where an intermediarycoloured compound (HMF-TBA) is utilised. The optimum pH condition (pH = 4.0--4.2) forthe mobile phase to'preventoverlapping between the HMF peak and the peaks of theother compound (a and /3) that coeluted with the HMF andalso the optimum organic solvent content (3 % or 7 %)were established. This is a sensitive, quick and safe methodfor the determination of HMF in fluid milk.
Description4 páginas, 2 figuras, 3 tablas
Appears in Collections:(IF) Artículos
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