Please use this identifier to cite or link to this item:
|Statistics||SHARE CORE MendeleyBASE||
|Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE|
|dc.contributor.author||Astudillo, Alma M.||-|
|dc.contributor.author||Balboa, María A.||-|
|dc.identifier.citation||Journal of Immunology 184(7): 3857-3865 (2010)||es_ES|
|dc.description||9 páginas, 8 figuras, 1 tabla.||es_ES|
|dc.description.abstract||Stimulated human monocytes undergo an intense trafficking of arachidonic acid (AA) among glycerophospholipidclasses. Using HPLC coupled to electrospray ionization mass spectrometry, we have characterized changes in the levels of AA-containing phospholipid species in human monocytes. In resting cells, AA was found esterified into various molecular species of phosphatidylinositol (PI), choline glycerophospholipids (PCs), and ethanolamine glycerophospholipids (PEs). All major AA-containing PC and PI molecular species decreased in zymosan-stimulated cells; however, no PE molecular species was found to decrease. In contrast, the levels of three AA-containing species increased in zymosan-activated cells compared with resting cells: 1,2-diarachidonyl-glycero-3-phosphoinositol [PI(20:4/20:4)]; 1,2-diarachidonyl-glycero-3-phosphocholine [PC(20:4/20:4)]; and 1-palmitoleoyl-2-arachidonyl-glycero-3-phosphoethanolamine [PE(16:1/20:4)]. PI(20:4/20:4) and PC(20:4/20:4), but not PE(16:1/20:4), also significantly increased when platelet-activating factor or PMA were used instead of zymosan to stimulate the monocytes. Analysis of the pathways involved in the synthesis of these three lipids suggest that PI(20:4/20:4) and PC(20:4/20:4) were produced in a deacylation/reacylation pathway via acyl-CoA synthetase–dependent reactions, whereas PE(16:1/20:4) was generated via a CoA-independent transacylation reaction. Collectively, our results define the increases in PI(20:4/20:4) and PC(20:4/20:4) as lipid metabolic markers of human monocyte activation and establish lipidomics as a powerful tool for cell typing under various experimental conditions.||es_ES|
|dc.description.sponsorship||This work was supported by the Spanish Ministry of Science and Innovation (Grants BFU2007-67154 and SAF2007-60055) and by the Regional Government of Castile and León (Grant CSI09-A08). D.B. was supported by predoctoral fellowships from Fundación Mario Losantos del Campo and Plan de Formación de Profesorado Universitario (Spanish Ministry of Science and Innovation). A.M.A. was supported by a predoctoral fellowship from the Regional Government of Castile and León.||es_ES|
|dc.publisher||American Association of Immunologists||es_ES|
|dc.title||Markers of Monocyte Activation Revealed by Lipidomic Profiling of Arachidonic Acid-Containing Phospholipids||es_ES|
|Appears in Collections:||(IBGM) Artículos|
Files in This Item:
There are no files associated with this item.
Show simple item record
WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.