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dc.contributor.authorBalgoma, David-
dc.contributor.authorAstudillo, Alma M.-
dc.contributor.authorPérez-Chacón, Gema-
dc.contributor.authorMontero, Olimpio-
dc.contributor.authorBalboa, María A.-
dc.contributor.authorBalsinde, Jesús-
dc.identifier.citationJournal of Immunology 184(7): 3857-3865 (2010)es_ES
dc.description9 páginas, 8 figuras, 1 tabla.es_ES
dc.description.abstractStimulated human monocytes undergo an intense trafficking of arachidonic acid (AA) among glycerophospholipidclasses. Using HPLC coupled to electrospray ionization mass spectrometry, we have characterized changes in the levels of AA-containing phospholipid species in human monocytes. In resting cells, AA was found esterified into various molecular species of phosphatidylinositol (PI), choline glycerophospholipids (PCs), and ethanolamine glycerophospholipids (PEs). All major AA-containing PC and PI molecular species decreased in zymosan-stimulated cells; however, no PE molecular species was found to decrease. In contrast, the levels of three AA-containing species increased in zymosan-activated cells compared with resting cells: 1,2-diarachidonyl-glycero-3-phosphoinositol [PI(20:4/20:4)]; 1,2-diarachidonyl-glycero-3-phosphocholine [PC(20:4/20:4)]; and 1-palmitoleoyl-2-arachidonyl-glycero-3-phosphoethanolamine [PE(16:1/20:4)]. PI(20:4/20:4) and PC(20:4/20:4), but not PE(16:1/20:4), also significantly increased when platelet-activating factor or PMA were used instead of zymosan to stimulate the monocytes. Analysis of the pathways involved in the synthesis of these three lipids suggest that PI(20:4/20:4) and PC(20:4/20:4) were produced in a deacylation/reacylation pathway via acyl-CoA synthetase–dependent reactions, whereas PE(16:1/20:4) was generated via a CoA-independent transacylation reaction. Collectively, our results define the increases in PI(20:4/20:4) and PC(20:4/20:4) as lipid metabolic markers of human monocyte activation and establish lipidomics as a powerful tool for cell typing under various experimental conditions.es_ES
dc.description.sponsorshipThis work was supported by the Spanish Ministry of Science and Innovation (Grants BFU2007-67154 and SAF2007-60055) and by the Regional Government of Castile and León (Grant CSI09-A08). D.B. was supported by predoctoral fellowships from Fundación Mario Losantos del Campo and Plan de Formación de Profesorado Universitario (Spanish Ministry of Science and Innovation). A.M.A. was supported by a predoctoral fellowship from the Regional Government of Castile and León.es_ES
dc.publisherAmerican Association of Immunologistses_ES
dc.titleMarkers of Monocyte Activation Revealed by Lipidomic Profiling of Arachidonic Acid-Containing Phospholipidses_ES
dc.description.peerreviewedPeer reviewedes_ES
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