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Title

Synthesis and properties of small interfering RNA duplexes carrying 5-ethyluridine residues

AuthorsTerrazas, Montserrat ; Eritja Casadellà, Ramón
KeywordsSiRNA
RNA interference
5-Ethyluridine
Dual luciferase assay
Oligonucleotide synthesis
Solid-phase
Issue DateAug-2011
PublisherSpringer
CitationMolecular Diversity 15(3): 677-686 (2011)
AbstractOligoribonucleotides carrying 5-ethyluridine units were prepared using solid-phase phosphoramidite chemistry. The introduction of the tert-butyldimethylsilyl group at the 2′-OH position proceeded in good yield and very high 2′-regioselectivity. RNA duplexes carrying 5-ethyluridine either at the sense or the guide strands display RNAi activity comparable to or slightly better than that of unmodified RNA duplexes. Gene suppression experiments using luciferase targets in SH-SY5Y cells show that the ethyl group is generally well accepted at all positions although a small decrease in RNA interference activity is observed when one 5-ethylU residue is incorporated in the 3′ overhangs.
Publisher version (URL)http://dx.doi.org/10.1007/s11030-010-9290-1
URIhttp://hdl.handle.net/10261/49294
DOI10.1007/s11030-010-9290-1
ISSN1381-1991
E-ISSN1573-501X
Appears in Collections:(IQAC) Artículos
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