English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/49269
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


Identification of european hake species (Merluccius merluccius) using real-time PCR

AuthorsSánchez, Ana Cristina ; Quinteiro, Javier; Rey Méndez, Manuel; Pérez Martín, Ricardo Isaac ; González Sotelo, Carmen
KeywordsEuropean hake
Species identification
Real-time PCR
Merluccius merluccius
Control region
Issue Date2009
PublisherAmerican Chemical Society
CitationJournal of Agricultural and Food Chemistry 57(9): 3397-3403 (2009)
AbstractA rapid and precise method for identifying European hake (Merluccius merluccius) based on TaqMan technology is presented. The method can be applied to fresh, frozen, and processed fish products to detect the fraudulent or unintentional mislabeling of this species. Specific primers and a minor groove binding (MGB) TaqMan probe were designed for this purpose based on partial sequences of the mitochondrial DNA control region. Combinations of primers and probe concentrations that gave the lowest Ct value and the highest final fluorescence value were selected to carry out efficiency, specificity, and cross-reactivity assays. The method was successfully tested on 31 commercial hake samples. A Ct value of about 16 was obtained when Merluccius merluccius was present; however, the fluorescence signal was not detected most of the time (Ct value 40) or presented significantly higher Ct values (38.2 ± 0.96) for the nonhake species
Description7 páginas, 3 figuras, 4 tablas
Publisher version (URL)http://dx.doi.org/10.1021/jf8036165
Appears in Collections:(IIM) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.