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Title

Identification of european hake species (Merluccius merluccius) using real-time PCR

AuthorsSánchez, Ana Cristina ; Quinteiro, Javier; Rey Méndez, Manuel; Pérez Martín, Ricardo Isaac ; González Sotelo, Carmen
KeywordsEuropean hake
Species identification
Real-time PCR
Merluccius merluccius
Control region
Issue Date2009
PublisherAmerican Chemical Society
CitationJournal of Agricultural and Food Chemistry 57(9): 3397-3403 (2009)
AbstractA rapid and precise method for identifying European hake (Merluccius merluccius) based on TaqMan technology is presented. The method can be applied to fresh, frozen, and processed fish products to detect the fraudulent or unintentional mislabeling of this species. Specific primers and a minor groove binding (MGB) TaqMan probe were designed for this purpose based on partial sequences of the mitochondrial DNA control region. Combinations of primers and probe concentrations that gave the lowest Ct value and the highest final fluorescence value were selected to carry out efficiency, specificity, and cross-reactivity assays. The method was successfully tested on 31 commercial hake samples. A Ct value of about 16 was obtained when Merluccius merluccius was present; however, the fluorescence signal was not detected most of the time (Ct value 40) or presented significantly higher Ct values (38.2 ± 0.96) for the nonhake species
Description7 páginas, 3 figuras, 4 tablas
Publisher version (URL)http://dx.doi.org/10.1021/jf8036165
URIhttp://hdl.handle.net/10261/49269
DOI10.1021/jf8036165
ISSN0021-8561
E-ISSN1520-5118
Appears in Collections:(IIM) Artículos
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