English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/49244
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Activation of STIM1-Orai1 Involves an Intramolecular Switching Mechanism

AuthorsKorzeniowski, Marek K.; Manjarrés, Isabel M.
Issue DateNov-2010
PublisherAmerican Association for the Advancement of Science
CitationScience Signaling 3(148): ra82 (2010)
AbstractStromal interaction molecule 1 (STIM1) stimulates calcium ion (Ca2+) entry through plasma membrane Orai1 channels in response to decreased Ca2+ concentrations in the endoplasmic reticulum lumen. We identified an acidic motif within the STIM1 coiled-coil region that keeps its Ca2+ activation domain [Ca2+ release–activated Ca2+ (CRAC) activation domain/STIM1-Orai activating region (CAD/SOAR)]—a cytoplasmic region required for its activation of Orai1—inactive. The sequence of the STIM1 acidic motif shows substantial similarity to that of the carboxyl-terminal coiled-coil segment of Orai1, which is the postulated site of interaction with STIM1. Mutations within this acidic region rendered STIM1 constitutively active, whereas mutations within a short basic segment of CAD/SOAR prevented Orai1 activation. We propose that the CAD/SOAR domain is released from an intramolecular clamp during STIM1 activation, allowing the basic segment to activate Orai1 channels. This evolutionarily conserved mechanism of STIM1 activation resembles the regulation of protein kinases by intramolecular silencing through pseudosubstrate binding.
Descriptionet al.
Publisher version (URL)http://dx.doi.org/10.1126/scisignal.2001122
URIhttp://hdl.handle.net/10261/49244
DOI10.1126/scisignal.2001122
ISSN1945-0877
E-ISSN1937-9145
Appears in Collections:(IBGM) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.