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In vitro properties of small ruminant lentivirus genotype E

AuthorsJuganaru, Maria Magdalena; Reina, Ramsés ; Bertolotti, Luigi; Stella, Maria Cristina; Profiti, Margherita; Armentano, María G.; Bollo, Enrico; Amorena Zabalza, Beatriz ; Rosati, Sergio
KeywordsSmall ruminant lentivirus
Genotype E
Viral pseudotype
Entry assay
Issue DateFeb-2011
PublisherAcademic Press
CitationVirology 410(1): 88-95 (2011)
AbstractSmall ruminant lentivirus genotype E lacks the dUTPase subunit and vpr-like gene. Two strains (Roccaverano and Seui) with identical genetic organization have been described, with the env HV1–HV2 domains being the most divergent. Although dUTPase and vpr-like deletions have been involved in the RT fidelity in non dividing cells, both strains were able to replicate efficiently in blood derived macrophages (BDM), while virus production of E1 subtype was reduced or abrogated in replicating fibroblastic-like cells. The transcriptional activity of genotype E was similar in these two cellular populations. When viral pseudotypes were generated with the env of both viruses, Roccaverano pseudotype displayed a paranuclear localization on BDM, suggesting a different mechanism of entry. Polymorphic GAS and TAS sites in the U3 region, further suggest that a population different from classically activated macrophages can be infected by these viruses, opening new insights into lentiviruses with low or null pathogenic potential.
Description8 p., 6 figures, 1 table and references
Publisher version (URL)http://dx.doi.org/10.1016/j.virol.2010.10.031
Appears in Collections:(IDAB) Artículos
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