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http://hdl.handle.net/10261/48907
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Campo, Irene del | - |
dc.contributor.author | Ruiz, Raúl | - |
dc.contributor.author | Cuevas, Ana | - |
dc.contributor.author | Revilla, Carlos | - |
dc.contributor.author | Vielva, Luis | - |
dc.contributor.author | Cruz, Fernando de la | - |
dc.date.accessioned | 2012-04-25T10:16:59Z | - |
dc.date.available | 2012-04-25T10:16:59Z | - |
dc.date.issued | 2012-03 | - |
dc.identifier.citation | Plasmid 67(2): 174–182 (2012) | es_ES |
dc.identifier.issn | 0147-619X | - |
dc.identifier.uri | http://hdl.handle.net/10261/48907 | - |
dc.description | A special issue on chromosome dynamics in memory of Prof. Kurt Nordstrom. | es_ES |
dc.description.abstract | A cytometric method for the estimation of end-point conjugation rates is developed and adapted to surface conjugation. This method improves the through-put of conjugation assays based on replica-plating and results in less noisy experimental data. Although conjugation on solid surfaces deviates from ideal conditions in which cells are continuously mixed, results show that, within the limits of high initial population densities and short mating times, end-point estimates of the conjugation rates are robust measurements. They are independent of the donor/recipient ratios and, to some extent, of the sampling time. Remixing the mating population in the course of a conjugation experiment results in a boost in the frequency of transconjugants. | es_ES |
dc.description.sponsorship | This work was supported by Grants BFU2008-00995/BMC from Ministerio de Ciencia e Innovación (MCINN, Spain), RD06/0008/1012 from Instituto de Salud Carlos III, and 248919/FP7-ICT-2009-4 from the European VII Framework Program. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/248919 | - |
dc.rights | closedAccess | es_ES |
dc.subject | Bacterial conjugation | es_ES |
dc.subject | Surface mating | es_ES |
dc.subject | Flow-cytometer | es_ES |
dc.subject | GFP | es_ES |
dc.title | Determination of conjugation rates on solid surfaces | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1016/j.plasmid.2012.01.008 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.plasmid.2012.01.008 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
item.languageiso639-1 | en | - |
Aparece en las colecciones: | (IBBTEC) Artículos |
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accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
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