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Determination of conjugation rates on solid surfaces

AuthorsCampo, Irene del ; Ruiz, Raúl ; Cuevas, Ana ; Revilla, Carlos ; Vielva, Luis; Cruz, Fernando de la
KeywordsBacterial conjugation
Surface mating
Issue DateMar-2012
CitationPlasmid 67(2): 174–182 (2012)
AbstractA cytometric method for the estimation of end-point conjugation rates is developed and adapted to surface conjugation. This method improves the through-put of conjugation assays based on replica-plating and results in less noisy experimental data. Although conjugation on solid surfaces deviates from ideal conditions in which cells are continuously mixed, results show that, within the limits of high initial population densities and short mating times, end-point estimates of the conjugation rates are robust measurements. They are independent of the donor/recipient ratios and, to some extent, of the sampling time. Remixing the mating population in the course of a conjugation experiment results in a boost in the frequency of transconjugants.
DescriptionA special issue on chromosome dynamics in memory of Prof. Kurt Nordstrom.
Publisher version (URL)http://dx.doi.org/10.1016/j.plasmid.2012.01.008
Appears in Collections:(IBBTEC) Artículos
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