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Title

Label-free voltammetric immunosensor using a nanoporous membrane based platform

AuthorsEscosura-Muñiz, Alfredo de la ; Merkoçi, Arben
KeywordsNanopores
Nanochannels
Immunosensor
Label-free
Screen-printed electrodes
Voltammetric detection
Issue DateJun-2010
PublisherElsevier
CitationElectrochemistry Communications 12(6): 859-863 (2010)
AbstractA novel device and methodology for the rapid and simple label-free electrochemical detection of proteins based on screen-printed carbon electrodes (SPCEs) modified with nanoporous Al2O3 membranes is reported. The nanoporous membranes are functionalized with antibodies and followed by the immunorecognition event that gives rise to the pore blocking. The blockage inside the nanochannels is fast, pore size dependent and easy to be detected by measuring the decrease in the differential pulse voltammetric (DPV) peak current of the [Fe(CN6)]4−/3− redox specie used as indicator. The developed nanoporous membrane based device represents a simple biodetection alternative that can be extended in the future to several other immuno and DNA detection systems.
Description5 páginas, 3 figuras.
Publisher version (URL)http://dx.doi.org/10.1016/j.elecom.2010.04.007
URIhttp://hdl.handle.net/10261/48740
DOI10.1016/j.elecom.2010.04.007
ISSN1388-2481
Appears in Collections:(CIN2) Artículos
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