English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/48725
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Brain CYP1A in seabream, Sparus aurata exposed to Benzo(a)pyrene

AuthorsOrtiz-Delgado, Juan B. ; Segner, Helmut; Sarasquete, Carmen
KeywordsSparus aurata
Larvae
Adult
Brain
CYP1A
EROD
B(a)P
Issue Date2009
PublisherUniversidad de Murcia
CitationHistology and Histopathology (Cellular and Molecular Biology ) 24(10): 1263-1273 (2009)
AbstractThis study compares basal and induced expression of cytochrome P4501A-CYP1A in the brain of gilthead seabream, Sparus aurata. Larval or adult seabream were exposed to benzo(a)pyrene -B(a)P- and the CYP1A response was assessed by analyzing CYP1A mRNA (RT-PCR), CYP1A protein (expression levels: ELISA, western blotting; cellular localization: immunohistochemistry), and CYP1A catalytic activity (7-ethoxyresorufin-O-deethylase-EROD). In the brain of adult S. aurata, CYP1A immunostaining was generally detected in the vasculature. It was present in the neuronal fibers and glial cells of the olfactory bulbs and the ventral telencephalon. ELISA and RT-PCR analyses confirmed CYP1A expression in the brains of non-exposed seabream. B(a)P exposure led to increased CYP1A staining mainly in neuronal fibers and glial cells of the olfactory bulbs, but also in the vascular endothelia. EROD activity, however, could not be detected in the brain of adult seabream, neither in control nor in exposed fish. In the developing brain of S. aurata larvae, immunohistochemical staining detected CYP1A protein exclusively in endothelia of the olfactory placode and in retina. Staining intensity of CYP1A slightly increases with larval development, especially in vascular brain endothelia. Exposing the larvae to 0.3 or 0.5 µg B(a)P/L from hatching until 15 days post hatching (dph) did not result in enhanced CYP1A immunostaining in the brain. In samples of whole seabream larvae, both from controls and BaP treatments, neither CYP1A mRNA, protein nor catalytic activity were detectable. The results demonstrate that CYP1A is expressed already and inducible in the larval brain, but that the regional and cellular expression differs partly between larval and adult brain. This may have implications for the toxicity of CYP1A-inducing xenobiotics on early and mature life stages of seabream.
Description11 páginas, 5 figuras.
Publisher version (URL)http://www.hh.um.es/Abstracts/Vol_24/24_10/24_10_1263.htm
URIhttp://hdl.handle.net/10261/48725
ISSN0213-3911
E-ISSN1699-5848
Appears in Collections:(ICMAN) Artículos
Files in This Item:
File Description SizeFormat 
OrtizDElgado2009BAPSEabream.pdf1,71 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.