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Improving genetic transformation of European chestnut and cryopreservation of transgenic lines

AuthorsCorredoira, Elena ; San José, M. Carmen; Viéitez Martín, Ana María ; Ballester, Antonio
Castanea sativa
Embryogenic lines
Issue Date2007
CitationPlant Cell Tissue and Organ Culture 91: 281-288 (2007)
AbstractThe aim of the present work was to study the effect of the developmental stage of the somatic embryos and of the genotype on the genetic transformation of embryogenic lines of European chestnut (Castanea sativa Mill.) and the cryopreservation of the embryogenic lines that are generated. As an initial source of explants in the transformation experiments, it was found that the use of somatic embryos isolated in the globular stage or clumps of 2-3 embryos in globular/ heart-shaped stages was more effective (30%) than when embryos at the cotyledonary stage were used (6.7%). All of the seven genotypes tested were transformed, and transformation efficiency was clearly genotype dependent. Three transgenic lines were successfully cryopreserved using the vitrification procedure, and the stable integration of the uidA gene into the transgenic chestnut plants that were regenerated subsequent to cryopreservation was demonstrated.
Publisher version (URL)http://dx.doi.org/10.1007/s11240-007-9295-3
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