English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/48696
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Characterization and expression of a Pinus radiata putative ortholog to the Arabidopsis SHORT-ROOT gene

AutorSolé, Alicia; Sánchez Fernández, M.ª Concepción; Vielba, Jesús; Valladares, Silvia; Abarca, Dolores; Díaz-Sala, Carmen
Palabras claveadventitious rooting
GRAS genes
pine
root meristem
vegetative propagation
Fecha de publicación2008
EditorHeron Publishing
CitaciónTree Physiology 28 (11): 1629–1639 (2008)
ResumenWe characterized a Pinus radiata D. Don putative ortholog to the Arabidopsis thaliana (L.) Heynh. SHORT-ROOT gene (AtSHR) and analyzed its expression in different organs during vegetative development and in response to exogenous auxin during adventitious rooting. The predicted protein sequence contained domains characteristic of the GRAS protein family and showed a strong similarity to the SHORT-ROOT (SHR) proteins. Quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) and in situ hybridization showed that the gene is predominantly expressed in roots, root primordia and in the cambial region of hypocotyl cuttings. Increased mRNA levels were observed, independently of the presence or absence of exogenous auxin, in the cambial region and rooting competent cells of hypocotyl cuttings within the first 24 h of adventitious rooting, before the activation of cell divisions and the organization of the adventitious root meristem. The expression pattern in organs and during adventitious rooting was similar to that of a Pinus radiata SCARECROW-LIKE (PrSCL1) gene, except that PrSCL1 is induced in response to exogenous auxin. Results suggest that the Pinus radiata SHORT-ROOT (PrSHR) gene has a role in root meristem formation and maintenance and in the cambial region of hypocotyl cuttings.
Versión del editorhttp://dx.doi.org/10.1093/treephys/28.11.1629
URIhttp://hdl.handle.net/10261/48696
DOI10.1093/treephys/28.11.1629
ISSN0829-318X
E-ISSN1758-4469
Aparece en las colecciones: (IIAG) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Characterization....pdf1,52 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.