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Title

Genome-wide screening of genes whose enhanced expression affects glycogen accumulation in escherichia coli

AuthorsEydallin, Gustavo ; Montero, Manuel ; Almagro, Goizeder ; Sesma, María Teresa ; Viale, Alejandro M. ; Muñoz Pérez, Francisco José ; Rahimpour, Mehdi ; Baroja-Fernández, Edurne ; Pozueta Romero, Javier
KeywordsASKA library
Carbohydrate metabolism
Functional genomics
Issue DateApr-2010
PublisherOxford University Press
CitationDNA Research 17(2): 61-71 (2010)
AbstractUsing a systematic and comprehensive gene expression library (the ASKA library), we have carried out a genome-wide screening of the genes whose increased plasmid-directed expression affected glycogen metabolism in Escherichia coli. Of the 4123 clones of the collection, 28 displayed a glycogen-excess phenotype, whereas 58 displayed a glycogen-deficient phenotype. The genes whose enhanced expression affected glycogen accumulation were classified into various functional categories including carbon sensing, transport and metabolism, general stress and stringent responses, factors determining intercellular communication, aggregative and social behaviour, nitrogen metabolism and energy status. Noteworthy, one-third of them were genes about which little or nothing is known. We propose an integrated metabolic model wherein E. coli glycogen metabolism is highly interconnected with a wide variety of cellular processes and is tightly adjusted to the nutritional and energetic status of the cell. Furthermore, we provide clues about possible biological roles of genes of still unknown functions.
Description11 p., 3 figures, 2 tables and bibliography
Publisher version (URL)http://dx.doi.org/10.1093/dnares/dsp028
URIhttp://hdl.handle.net/10261/48680
DOI10.1093/dnares/dsp028
ISSN1340-2838
E-ISSN1756-1663
Appears in Collections:(IDAB) Artículos
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