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DNA hybridization detection by electrochemical impedance spectroscopy using interdigitated gold nanoelectrodes

AuthorsBonanni, Alessandra; Fernández-Cuesta, Irene; Borrisé, Xavier ; Perez Murano, Francesc X. ; Alegret, Salvador; Valle, Manel del
Interdigitated electrodes
Impedimetric genosensor
Gold nanoparticle amplification
BRCA1 gene
Issue Date2010
CitationMicrochimica Acta 170(3-4): 275-281 (2010)
AbstractA DNA biosensor is presented that is based on gold interdigitated nanoelectrodes of 100 nm width and 250 nm pitch. A single-strand oligonucleotide acts as the capture probe. Because of its nanometer dimensions, the device shows improved sensitivity when compared to similar systems. This encouraged us to perform a direct and unlabelled detection. After incubation with the DNA target, the impedance spectrum was recorded between 1 kHz and 10 MHz to obtain the net capacitance change. The use of a biotinylated DNA signalling probe permitted the integration of an amplification stage in a sandwich format that employs streptavidin-modified gold nanoparticles. The strategy was preliminarily tested by detecting the breast cancer related BRCA1 gene, where the noncomplementary, wild and mutated forms were easily differentiated at a concentration level of 3 μM (corresponding to a 30 pmol quantity).
Description7 páginas, 5 figuras, 1 tabla.
Publisher version (URL)http://dx.doi.org/10.1007/s00604-010-0358-5
Appears in Collections:(IMB-CNM) Artículos
(CIN2) Artículos
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