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Título

Selective inhibition of clade A PP2Cs by PYR/PYLs

AutorAntoni, Regina ; González-Guzmán, Miguel; Rodríguez, Lesia ; Rodrigues, Americo do Patrocinio ; Pizzio, Gastón A.; Rodríguez, Pedro L.
Palabras claveAnion Channel SLAC1
Protein-kinases
Negative regulator
Stress responses
Fecha de publicaciónfeb-2012
EditorAmerican Society of Plant Biologists
CitaciónPlant Physiology 158/2:970-980 (2012)
ResumenClade A protein phosphatases type 2C (PP2Cs) are negative regulators of abscisic acid (ABA) signalling that are inhibited in an ABA-dependent manner by PYRABACTIN RESISTANCE1 (PYR1)/PYR1-LIKE (PYL)/ REGULATORY COMPONENTS OF ABA RECEPTORS (RCAR) intracellular receptors. We provide genetic evidence that a previously uncharacterized member of this PP2C family in Arabidopsis thaliana, At5g59220, is a negative regulator of osmotic stress and ABA signalling and this function was only apparent when double loss-of-function mutants with pp2ca-1/ahg3 were generated. At5g59220-GFP and its close relative PP2CA-GFP showed a predominant nuclear localization, however, hemagglutinin (HA)-tagged versions were also localized to cytosol and microsomal pellets. At5g59220 was selectively inhibited by some PYR/PYL ABA receptors, and close relatives of this PP2C, such as PP2CA/ABA-HYPERSENSITIVE GERMINATION3 (AHG3) and AHG1, showed a contrasting sensitivity to PYR/PYL inhibition. Interestingly, AHG1 was resistant to inhibition by the PYR/PYL receptors tested, which suggests that this seed-specific phosphatase is still able to regulate ABA signalling in the presence of ABA and PYR/PYL receptors and therefore to control the highly active ABA signalling pathway that operates during seed development. Moreover, the differential sensitivity of the phosphatases At5g59220 and PP2CA to inhibition by ABA receptors reveals a functional specialization of PYR/PYL ABA receptors to preferentially inhibit certain PP2Cs.
URIhttp://hdl.handle.net/10261/48606
DOI10.1104/pp.111.188623
E-ISSN0032-0889
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