English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/48580
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Aptamers based electrochemical biosensor for protein detection using carbon nanotubes platforms

AuthorsKara, Pinar; Escosura-Muñiz, Alfredo de la ; Maltez, Marisa ; Guix, María; Ozsoz, Mehmet; Merkoçi, Arben
KeywordsAptamer
Aptasensor
Thrombin
Electrochemical impedance spectroscopy
Screen-printed electrodes
Carbon nanotubes
Issue DateDec-2010
PublisherElsevier
CitationBiosensors and Bioelectronics 26(4): 1715-1718 (2010)
AbstractA label-free bioelectronic detection of aptamer–thrombin interaction based on electrochemical impedance spectroscopy (EIS) technique is reported. Multiwalled carbon nanotubes (MWCNTs) were used as modifiers of screen-printed carbon electrotransducers (SPCEs), showing improved characteristics compared to the bare SPCEs. 5′amino linked aptamer sequence was immobilized onto the modified SPCEs and then the binding of thrombin to aptamer sequence was monitored by EIS transduction of the resistance to charge transfer (Rct) in the presence of 5 mM [Fe(CN)6]3−/4−, obtaining a detection limit of 105 pM. This study represents an alternative electrochemical biosensor for the detection of proteins with interest for future applications.
Description4 páginas, 3 figuras.
Publisher version (URL)http://dx.doi.org/10.1016/j.bios.2010.07.090
URIhttp://hdl.handle.net/10261/48580
DOI10.1016/j.bios.2010.07.090
ISSN0956-5663
Appears in Collections:(CIN2) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.