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Microscopy studies reveal delignification and sterol removal from eucalypt kraft pulps by laccase-HBT

AuthorsSperanza, Mariela ; Ibarra, David ; Romero, Javier; Martínez, Ángel T. ; Martínez, María Jesús ; Camarero, Susana
eucalypt pulp
filipin staining
Issue Date2007
PublisherInforma Healthcare
CitationBiocatalysis and Biotransformation 25(2-4): 251-259(2007)
AbstractFungal laccases in the presence of mediators are powerful biocatalysts to degrade lignin. Pycnoporus cinnabarinus laccase and 1-hydroxybenzotriazole (HBT) have been successfully used to delignify eucalypt kraft pulp once integrated in a totally chlorine-free bleaching sequence. Real time delignification of kraft pulp by laccase–HBT was verified in situ by monitoring the loss of lignin autofluorescence during the enzymatic treatment using confocal laser scanning microscopy. The highest delignification of pulp fibers occurred over a very short time-span (5 min). Moreover, we demonstrate the removal of sterols, responsible for pitch deposits in hardwood kraft pulps, as an additional effect of laccase-HBT. Spherical structures between pulp fibers localized by low temperature scanning electron microscopy were removed by laccase–HBT. The use of filipin, a specific stain, revealed the sterol nature of many of these structures. At the end of the enzyme-aided bleaching sequence, the fluorescent sterols–filipin signals were almost completely absent
Description9 páginas, 6 figuras -- PAGS nros. 251-259
Publisher version (URL)http://informahealthcare.com/doi/abs/10.1080/10242420701444348
Appears in Collections:(CIB) Artículos
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