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Kinetics of SH-ssDNA immobilization on gold coated cantilevers under electrochemical control

AuthorsArroyo-Hernández, María ; Martín-González, Marisol; Costa Krämer, José Luis
Reaction kinetics
Issue DateNov-2010
PublisherJohn Wiley & Sons
Citationphysica status solidi (c) 7(11-12) 2770-2773 (2010)
AbstractControlling electrochemical parameters (setting the bias voltage and monitoring the current) during immobilization of thiolated single stranded DNA (SH-ssDNA) on gold coated cantilevers allows tailoring the reaction kinetics. In particular, the immobilization time can be reduced more than 40 times when compared with conventional passive immobilization (cantilever electrically floated during the process). Besides the kinetics, the surface stress induced during the immobilization process can be also controlled and maximized. The combination of an electrochemically monitored immobilization with in-situ profile measurements provides a powerful tool to optimize DNA immobilization and increase the nanomechanical response.
Publisher version (URL)http://dx.doi.org/10.1002/pssc.200983819
Appears in Collections:(IMN-CNM) Artículos
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