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Title

Identification of commercial prawn and shrimp species of food interest by native isoelectric focusing

AuthorsOrtea, Ignacio ; Cañas, Benito; Calo-Mata, Pilar ; Barros-Velázquez, J. ; Gallardo, José Manuel
KeywordsIEF
Shrimps
Species identification
SCP
MS/MS
Issue Date2010
PublisherElsevier
CitationFood Chemistry 121(2): 569-574 (2010)
AbstractNative isoelectric focusing (IEF) of water-soluble sarcoplasmic proteins was applied to the identification of 14 shrimp species of food interest belonging to the order Decapoda. These species have different commercial values, but due to their phenotypic similarities and carapace removal in their industrial processing, incorrect food labelling and deliberate or inadvertent adulteration can occur. Each of the 14 tested species showed species-specific protein band profiles and intra-specific polymorphism was low, not preventing the correct identification of the species. Therefore, IEF of water-soluble sarcoplasmic proteins allowed the differentiation of the 14 species considered. In addition, sarcoplasmic calcium-binding proteins (SCPs) were identified by tandem mass spectrometry (MS/MS) as the major species-specific proteins in these species, opening the way to further studies focusing on their potential use as specific biomarkers
Description6 páginas, 3 figuras, 3 tablas
Publisher version (URL)http://dx.doi.org/10.1016/j.foodchem.2009.12.049
URIhttp://hdl.handle.net/10261/48265
DOI10.1016/j.foodchem.2009.12.049
ISSN0308-8146
Appears in Collections:(IIM) Artículos
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