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Title

Rapid direct detection of the major fish allergen, parvalbumin, by selected MS/MS Ion Monitoring mass spectrometry

AuthorsCarrera, Mónica ; Cañas, Benito; Gallardo, José Manuel
KeywordsAllergen
Proteomics
Selected MS/MS ion monitoring (SMIM)
High intensity focused ultrasound (HIFU)
Mass spectrometry
Mass spectrometry
PRVB
Fish
Food
Marine
Issue Date2012
PublisherElsevier
CitationJournal of Proteomics 75(11): 3211-3220 (2012)
AbstractParvalbumins beta (β-PRVBs) are considered the majorfishallergens. A new strategy for the rapid and directdetection of these allergens in any foodstuff is presented in this work. The proposed methodology is based on the purification of β-PRVBs by treatment with heat, the use of accelerated in-solution trypsin digestion under an ultrasonic field provided by High-Intensity Focused Ultrasound (HIFU) and the monitoring of only nineteen β-PRVB peptide biomarkers by Selected MS/MS Ion Monitoring (SMIM) in a linear ion trap (LIT) mass spectrometer. The present strategy allows the directdetection of the presence of fish β-PRVBs in any food product in less than 2 hours. This new affordable assay would be very useful for the sanitary and inspect authorities to protect sensitive consumers from allergic reactions and to guarantee their safety
Description10 páginas, 5 figuras, 2 tablas
Publisher version (URL)http://dx.doi.org/10.1016/j.jprot.2012.03.030
URIhttp://hdl.handle.net/10261/48110
DOI10.1016/j.jprot.2012.03.030
ISSN1874-3919
Appears in Collections:(IIM) Artículos
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