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Title

Phylogenetic characterization and in situ detection of bacterial communities associated with seahorses (Hippocampus guttulatus) in captivity

AuthorsBalcázar, José Luis ; Lee, Natuschka; Pintado, José ; Planas, Miguel
KeywordsSeahorses
Captivity conditions
Bacterial community structure
Clone libraries
Fluorescence in situ hybridization
Issue Date2010
PublisherElsevier
CitationSystematic and Applied Microbiology 33(2): 71-77 (2010)
AbstractAlthough there are several studies describing bacteria associated with marine fish, the bacterial composition associated with seahorses has not been extensively investigated since these studies have been restricted to the identification of bacterial pathogens. In this study, the phylogenetic affiliation of seahorse-associated bacteria was assessed by 16S rRNA gene sequencing of cloned DNA fragments. Fluorescence in situ hybridization (FISH) was used to confirm the presence of the predominant groups indicated by 16S rRNA analysis. Both methods revealed that Vibrionaceae was the dominant population in Artemia sp. (live prey) and intestinal content of the seahorses, while Rhodobacteraceae was dominant in water samples from the aquaculture system and cutaneous mucus of the seahorses. To our knowledge, this is the first time that bacterial communities associated with healthy seahorses in captivity have been described
Description2 tablas, 1 figura
Publisher version (URL)http://dx.doi.org/10.1016/j.syapm.2009.11.005
URIhttp://hdl.handle.net/10261/48085
DOI10.1016/j.syapm.2009.11.005
ISSN0723-2020,
E-ISSN1618-0984
Appears in Collections:(IIM) Artículos
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