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Ligands of boron in Pisum sativum nodules are involved in regulation of oxygen concentration and rhizobial infection

AuthorsReguera, María; Wimmer, Monika; Bustos-Sanmamed, Pilar CSIC ORCID; Goldbach, Einer E.; Bolaños, Luis; Bonilla, Ildefonso
KeywordsBorate complexes
legume AGP–extensin
legume rhizobin symbiosis
Issue DateJun-2010
PublisherJohn Wiley & Sons
CitationReguera M, Wimmer M, Bustos P, Goldbach EE, Bolaños L, Bonilla I. Ligands of boron in Pisum sativum nodules are involved in regulation of oxygen concentration and rhizobial infection. Plant Cell & Environment 33 (6): 1039-1048 (2010)
AbstractBoron (B) is an essential nutrient for N(2)-fixing legume-rhizobia symbioses, and the capacity of borate ions to bind and stabilize biomolecules is the basis of any B function. We used a borate-binding-specific resin and immunostaining techniques to identify B ligands important for the development of Pisum sativum- Rhizobium leguminosarum 3841 symbiotic nodules. arabinogalactan-extensin (AGPE), recognized by MAC 265 antibody, appeared heavily bound to the resin in extracts derived from B-sufficient, but not from Bdeficient nodules. MAC 265 stained the infection threads and the extracellular matrix of cortical cells involved in the oxygen diffusion barrier. In B-deprived nodules, immunolocalization of MAC 265 antigens was significantly reduced. Leghaemoglobin (Lb) concentration largely decreased in B-deficient nodules. The absence of MAC 203 antigens in B-deficient nodules suggests a high internal oxygen concentration, as this antibody detects an epitope on the lipopolysaccharide (LPS) of bacteroids typically expressed in microaerobically grown R. leguminosarum 3841. However, B-deprived nodules did not accumulate oxidized lipids and proteins, and revealed a decrease in the activity of the major antioxidant enzyme ascorbate peroxidase (APX). Therefore, B deficiency reduced the stability of nodule macromolecules important for rhizobial infection, and for regulation of oxygen concentration, resulting in non-functional nodules, but did not appear to induce oxidative damage in low-B nodules.
Description16 Pags., 7 Figs. The definitive version is available at: http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1365-3040
Publisher version (URL)http://dx.doi.org/10.1111/j.1365-3040.2010.02125.x
Appears in Collections:(EEAD) Artículos
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