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Título

Topo IV is the topoisomerase that knots and unknots sister duplexes during DNA replication

AutorLópez, Virginia CSIC ORCID; Martínez-Robles, María Luisa CSIC; Hernandez, Pablo CSIC ; Krimer, Dora B. CSIC ORCID ; Schvartzman, Jorge Bernardo CSIC ORCID
Palabras claveTopoisomerase IV
DNA replication
Sister duplexes
DNA knots
Fecha de publicación19-dic-2011
EditorOxford University Press
CitaciónNucleic Acids Res. 1-11(2011)
ResumenDNA topology plays a crucial role in all living cells. In prokaryotes, negative supercoiling is required to initiate replication and either negative or positive supercoiling assists decatenation. The role of DNA knots, however, remains a mystery. Knots are very harmful for cells if not removed efficiently, but DNA molecules become knotted in vivo. If knots are deleterious, why then does DNA become knotted? Here, we used classical genetics, high-resolution 2D agarose gel electrophoresis and atomic force microscopy to show that topoisomerase IV (Topo IV), one of the two type-II DNA topoisomerases in bacteria, is responsible for the knotting and unknotting of sister duplexes during DNA replication. We propose that when progression of the replication forks is impaired, sister duplexes become loosely intertwined. Under these conditions, Topo IV inadvertently makes the strand passages that lead to the formation of knots and removes them later on to allow their correct segregation.
Descripción11 páginas, 8 figuras, 1 tabla, -- PAGS nros. 1-11
Versión del editorhttp://dx.doi.org/10.1093/nar/gkr1237
URIhttp://hdl.handle.net/10261/47917
DOI10.1093/nar/gkr1237
ISSN0305-1048
E-ISSN1362-4962
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