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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Di Serio, Francesco | - |
dc.contributor.author | Gisel, Andreas | - |
dc.contributor.author | Navarro, Beatriz | - |
dc.contributor.author | Delgado, Sonia | - |
dc.contributor.author | Martínez de Alba, Ángel-Emilio | - |
dc.contributor.author | Donvito, Giacinto | - |
dc.contributor.author | Flores, Ricardo | - |
dc.date.accessioned | 2012-04-03T11:13:01Z | - |
dc.date.available | 2012-04-03T11:13:01Z | - |
dc.date.issued | 2009-10-21 | - |
dc.identifier.citation | Plos One 4/10:e7539 (2009) | es_ES |
dc.identifier.uri | http://hdl.handle.net/10261/47891 | - |
dc.description.abstract | Northern-blot hybridization and low-scale sequencing have revealed that plants infected by viroids, non-protein-coding RNA replicons, accumulate 21–24 nt viroid-derived small RNAs (vd-sRNAs) similar to the small interfering RNAs, the hallmarks of RNA silencing. These results strongly support that viroids are elicitors and targets of the RNA silencing machinery of their hosts. Low-scale sequencing, however, retrieves partial datasets and may lead to biased interpretations. To overcome this restraint we have examined by deep sequencing (Solexa-Illumina) and computational approaches the vdsRNAs accumulating in GF-305 peach seedlings infected by two molecular variants of Peach latent mosaic viroid (PLMVd) inciting peach calico (albinism) and peach mosaic. Our results show in both samples multiple PLMVd-sRNAs, with prevalent 21-nt (+) and (2) RNAs presenting a biased distribution of their 59 nucleotide, and adopting a hotspot profile along the genomic (+) and (2) RNAs. Dicer-like 4 and 2 (DCL4 and DCL2, respectively), which act hierarchically in antiviral defense, likely also mediate the genesis of the 21- and 22-nt PLMVd-sRNAs. More specifically, because PLMVd replicates in plastids wherein RNA silencing has not been reported, DCL4 and DCL2 should dice the PLMVd genomic RNAs during their cytoplasmic movement or the PLMVd-dsRNAs generated by a cytoplasmic RNA-dependent RNA polymerase (RDR), like RDR6, acting in concert with DCL4 processing. Furthermore, given that vd-sRNAs derived from the 12–14-nt insertion containing the pathogenicity determinant of peach calico are underrepresented, it is unlikely that symptoms may result from the accidental targeting of host mRNAs by vd-sRNAs from this determinant guiding the RNA silencing machinery. | es_ES |
dc.description.sponsorship | This work was supported by the Ministerio de Ciencia e Innovacio´n and the Generalidad Valenciana of Spain (BFU2008-03154/BMC and ACOMP/2009/ 151, respectively, to R.F.) and the Dipartimento Agroalimentare of the CNR of Italy (A. Leone and D. Mariotti 2008 award for advanced research in agriculture to F.D.S.). Funding for open access charge: Universidad Polite´cnica de Valencia. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Public Library of Science | es_ES |
dc.relation.isversionof | Publisher's version | - |
dc.rights | openAccess | es_ES |
dc.subject | Infected tomato plants | es_ES |
dc.subject | Dicer-like proteins | es_ES |
dc.subject | Silencing pathways | es_ES |
dc.subject | Self-cleavage | es_ES |
dc.title | Deep Sequencing of the Small RNAs Derived from Two Symptomatic Variants of a Chloroplastic Viroid: Implications for Their Genesis and for Pathogenesis | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1371/journal.pone.0007539 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.identifier.e-issn | 1932-6203 | - |
dc.identifier.pmid | 19847296 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.languageiso639-1 | en | - |
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Plos One 4_10_e7539.pdf | 955,88 kB | Adobe PDF | Visualizar/Abrir |
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