English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/47870
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Organization of two independent kisspeptin systems derived from evolutionary-ancient kiss genes in the brain of zebrafish

AuthorsServili, Arianna; Le Page, Yann; Leprince, Jérôme; Caraty, Alain; Escobar, Sebastián ; Parhar, Ishwar S.; Seong, Jae Yong; Vaudry, Hubert; Kah, Olivier
Issue DateApr-2011
PublisherEndocrine Society
CitationEndocrinology 152(4): 1527-1540 (2011)
AbstractKisspeptins are new actors in the neuroendocrine regulation of reproduction. In vertebrates, the number of kiss genes varies from none to three. Zebrafish have two kiss genes, kiss1 and kiss2, and two kiss receptors (GPR54), kiss1r and kiss2r. To provide detailed information on the organization of the kiss systems in zebrafish, antibodies were raised against the C terminus of zebrafish preproKiss1 and preproKiss2. Immunohistochemistry fully confirmed in situ hybridization data, showing that kiss1-expressing neurons are only located in the habenular nucleus, while kiss2-expressing neurons are found in the dorsal and ventral hypothalamus. Kiss1-expressing cells project only to the interpeduncular and raphe nuclei and strongly expressed the kiss1r receptor. In contrast, kiss2-expressing cells are mostly present in the dorsal and ventral hypothalamus and project widely into the subpallium, the preoptic area, the thalamus, the ventral and caudal hypothalamus, and the mesencephalon. All these regions strongly expressed the kiss2r messengers. Kiss2 fibers profusely innervate the ventral forebrain and notably made close apposition with GnRH3 neurons. Estrogen treatment of juvenile fish with estradiol causes increase in kiss2 and kiss2r expression. In the pituitary gland, no proKiss2-positive fibers were detected, while positive cells were observed in the pars intermedia. In addition to proposing a successful strategy to develop antibodies to kisspeptins, these data indicate that the kiss2 systems of zebrafish are implicated in reproductive events, while the kiss1 gene would play other functions that remain to be established.
Description14 p., 1 table, 8 figures and bibliography
Publisher version (URL)http://dx.doi.org/10.1210/en.2010-0948
URIhttp://hdl.handle.net/10261/47870
DOI10.1210/en.2010-0948
ISSN0013-7227
E-ISSN1945-7170
Appears in Collections:(IATS) Artículos
Files in This Item:
File Description SizeFormat 
Organization of two independent kisspeptin.pdf4,16 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.