Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/47837
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Topological complexity of different populations of pBR322 as visualized by two-dimensional agarose gel electrophoresis |
Autor: | Martin-Parras, Luis CSIC; Lucas, Isabelle A.; Martínez-Robles, María Luisa CSIC; Hernandez, Pablo CSIC ; Krimer, Dora B. CSIC ORCID ; Hyrien, Olivier; Schvartzman, Jorge Bernardo CSIC ORCID | Palabras clave: | Sv40 Dna-Replication Intertwined catenated Dimers Coli topoisomerase-I Processive recombination Potential origins Strand Exchange Xenopus Eggs |
Fecha de publicación: | 1998 | Editor: | Oxford University Press | Citación: | Nucleic Acids Res. 26(14):3424-3432(1998) | Resumen: | Neutral/neutral two-dimensional (2D) agarose gel electrophoresis was used to investigate populations of the different topological conformations that pBR322 can adopt in vivo in bacterial cells as well as in Xenopus egg extracts. To help in interpretation and identification of all the different signals, undigested as well as DNA samples pretreated with DNase I, topoisomerase I and topoisomerase II were analyzed. The second dimension of the 2D gel system was run with or without ethidium bromide to account for any possible changes in the migration behavior of DNA molecules caused by intercalation of this planar agent. Finally, DNA samples were isolated from a recA(-) strain of Escherichia coil, as well as after direct labeling of the replication intermediates in extracts of Xenopus laevis eggs. Altogether, the results obtained demonstrated that 2D gels can be readily used to identify most of the complex topological populations that circular molecules can adopt in vivo in both bacteria and eukaryotic cells. | Descripción: | 9 páginas, 5 figuras, -- PAGS nros. 3424-3432 | Versión del editor: | http://dx.doi.org/doi:10.1093/nar/26.14.3424 | URI: | http://hdl.handle.net/10261/47837 | DOI: | 10.1093/nar/26.14.3424 | ISSN: | 0305-1048 | E-ISSN: | 1362-4962 |
Aparece en las colecciones: | (CIB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
nar-1998.pdf | 259,7 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
25
checked on 05-abr-2024
SCOPUSTM
Citations
40
checked on 23-abr-2024
WEB OF SCIENCETM
Citations
37
checked on 21-feb-2024
Page view(s)
324
checked on 24-abr-2024
Download(s)
210
checked on 24-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.