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Título

Bi-directional replication and random termination

AutorSantamaria, David; Viguera, Enrique; Martínez-Robles, María Luisa ; Hyrien, Olivier; Hernandez, Pablo ; Krimer, Dora B. ; Schvartzman, Jorge Bernardo
Palabras claveNa-Replication
Escherichia-Coli
Saccharomyces-Cerevisiae
Plasmid Dna
Potential Origins
Topoisomerase-I
Xenopus Eggs
east Origin
Fecha de publicación2000
EditorOxford University Press
CitaciónNucleic Acids Res. 28(10),2099-2107(2000)
ResumenTwo-dimensional (2D) agarose gel electrophoresis was used to study termination of DNA replication in a shuttle vector, YRp7', when it replicated in Escherichia coli, Saccharomyces cerevisiae and Xenopus egg extracts. In E,coli, the 2D gel patterns obtained were consistent with uni-directional replication initiated at a specific site, the ColE1 origin, In consequence, termination also occurred precisely at the ColE1 origin, In Xenopus egg extracts, the particular shape of the bubble are as well as the triangular smear detected to the left of the simple-Y pattern indicated random initiation and termination. In S. cerevisiae, initiation occurred at the ARS1 origin and replication proceeded in a bi-directional manner, However, termination did not always occur at a specific site 180 degrees across from the origin, but almost all along the south hemisphere of the plasmid, Inversion, deletion or replacement of DNA sequences located throughout this hemisphere did not eliminate random termination. Analysis of the replication intermediates of another yeast plasmid bearing a different origin, ARS305, also exhibited random termination. We propose that the random termination events observed in S,cerevisiae could be due to an asynchronous departure of both forks from the bidirectional origin in addition to differences in the rate of fork progression. These observations could be extended to all bi-directional origins.
Descripción9 páginas, 8 figuras, -- PAGS nros. 2099-2107
Versión del editorhttp://dx.doi.org/10.1093/nar/28.10.2099
URIhttp://hdl.handle.net/10261/47746
DOI10.1093/nar/28.10.2099
ISSN0305-1048
E-ISSN0305-1048
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