Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/47743
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Olavarrieta, Leticia | - |
dc.contributor.author | Martínez-Robles, María Luisa | - |
dc.contributor.author | Sogo, José | - |
dc.contributor.author | Stasiak, Andrzej Z. | - |
dc.contributor.author | Hernandez, Pablo | - |
dc.contributor.author | Krimer, Dora B. | - |
dc.contributor.author | Schvartzman, Jorge Bernardo | - |
dc.date.accessioned | 2012-03-30T15:06:13Z | - |
dc.date.available | 2012-03-30T15:06:13Z | - |
dc.date.issued | 2002 | - |
dc.identifier.citation | Nucleic Acids Res. 30(3):656-666(2002) | es_ES |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.uri | http://hdl.handle.net/10261/47743 | - |
dc.description | 11 páginas, 8 figuras, -- PAGS nros.656-666, | es_ES |
dc.description.abstract | To study the structure of partially replicated plasmids, we cloned the Escherichia coli polar replication terminator TerE in its active orientation at different locations in the ColE1 vector pBR18. The resulting plasmids, pBR18-TerE@StyI and pBR18-TerE@EcoRI, were analyzed by neutral/neutral two-dimensional agarose gel electrophoresis and electron microscopy. Replication forks stop at the Ter-TUS complex, leading to the accumulation of specific replication intermediates with a mass 1.26 times the mass of non-replicating plasmids for pBR18-TerE@StyI and 1.57 times for pBR18-TerE@EcoRI. The number of knotted bubbles detected after digestion with ScaI and the number and electrophoretic mobility of undigested partially replicated topoisomers reflect the changes in plasmid topology that occur in DNA molecules replicated to different extents. Exposure to increasing concentrations of chloroquine or ethidium bromide revealed that partially replicated topoisomers (CCCRIs) do not sustain positive supercoiling as efficiently as their non-replicating counterparts. It was suggested that this occurs because in partially replicated plasmids a positive DeltaLk is absorbed by regression of the replication fork. Indeed, we showed by electron microscopy that, at least in the presence of chloroquine, some of the CCCRIs of pBR18-Ter@StyI formed Holliday-like junction structures characteristic of reversed forks. However, not all the positive supercoiling was absorbed by fork reversal in the presence of high concentrations of ethidium bromide. | es_ES |
dc.description.sponsorship | PM 9701-38 PGC PB98048 from the Spanish CICYT, 99/0850 from the Spanish FIS, 08.6/0016/2007 from CAM, and from the Swiss National Science Foundation to JMS (31-63418), and A.S. (31-61636)and (31-58841). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Oxford University Press | es_ES |
dc.rights | openAccess | es_ES |
dc.subject | Catenated dimers | es_ES |
dc.subject | Agarose-gel electrophoresis | es_ES |
dc.subject | Intertwined | es_ES |
dc.subject | SV40 DNA-replication | es_ES |
dc.subject | Escherichia-coli | es_ES |
dc.subject | Potential origins | es_ES |
dc.subject | Recombination | es_ES |
dc.subject | Termination | es_ES |
dc.title | Supercoiling, knotting and replication fork reversal in partially replicated plasmids | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1093/nar/30.3.656 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1093/nar/30.3.656 | es_ES |
dc.identifier.e-issn | 1362-4962 | - |
dc.identifier.pmid | 11809877 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.fulltext | With Fulltext | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.openairetype | artículo | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
Aparece en las colecciones: | (CIB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
nar-2002.pdf | 845,5 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
31
checked on 23-abr-2024
SCOPUSTM
Citations
58
checked on 15-abr-2024
WEB OF SCIENCETM
Citations
55
checked on 22-feb-2024
Page view(s)
323
checked on 24-abr-2024
Download(s)
239
checked on 24-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.