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dc.contributor.author | Aparicio Herrero, Frederic | - |
dc.contributor.author | Aparicio-Sanchís, Rafael | - |
dc.contributor.author | Gadea Vacas, José | - |
dc.contributor.author | Sánchez-Navarro, J. A. | - |
dc.contributor.author | Pallás Benet, Vicente | - |
dc.contributor.author | Murguía, José Ramón | - |
dc.date.accessioned | 2012-03-30T11:11:11Z | - |
dc.date.available | 2012-03-30T11:11:11Z | - |
dc.date.issued | 2011-11-08 | - |
dc.identifier.citation | Plos One 6/11:e27409 (2011) | es_ES |
dc.identifier.uri | http://hdl.handle.net/10261/47732 | - |
dc.description.abstract | Virus life cycle heavily depends on their ability to command the host machinery in order to translate their genomes. Animal viruses have been shown to interfere with host translation machinery by expressing viral proteins that either maintain or inhibit eIF2a function by phosphorylation. However, this interference mechanism has not been described for any plant virus yet. Prunnus necrotic ringspot virus (PNRSV) is a serious pathogen of cultivated stone fruit trees. The movement protein (MP) of PNRSV is necessary for the cell-to-cell movement of the virus. By using a yeast-based approach we have found that overexpression of the PNRSV MP caused a severe growth defect in yeast cells. cDNA microarrays analysis carried out to characterise at the molecular level the growth interference phenotype reported the induction of genes related to amino acid deprivation suggesting that expression of MP activates the GCN pathway in yeast cells. Accordingly, PNRSV MP triggered activation of the Gcn2p kinase, as judged by increased eIF2a phosphorylation. Activation of Gcn2p by MP expression required a functional Tor1p kinase, since rapamycin treatment alleviated the yeast cell growth defect and blocked eIF2a phosphorylation triggered by MP expression. Overall, these findings uncover a previously uncharacterised function for PNRSV MP viral protein, and point out at Tor1p and Gcn2p kinases as candidate susceptibility factors for plant viral infections. | es_ES |
dc.description.sponsorship | This work was supported by grants GV/2007/064 (Proyecto para grupos de investigacio´n emergentes) and (GV/2011/003) (Prometeo Project) from Conselleria d’Empresa, Universitat i Ciencia (Generalitat Valenciana). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Public Library of Science | es_ES |
dc.relation.isversionof | Publisher's version | - |
dc.rights | openAccess | es_ES |
dc.title | A Plant Virus Movement Protein Regulates the Gcn2p Kinase in Budding Yeast | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1371/journal.pone.0027409 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.identifier.e-issn | 1932-6203 | - |
dc.identifier.pmid | 22087310 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.fulltext | With Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.openairetype | artículo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
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PLOS ONE 6_e27409.pdf | 192,05 kB | Adobe PDF | Visualizar/Abrir |
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