Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/47731
COMPARTIR / EXPORTAR:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invitar a revisión por pares abierta
Campo DC Valor Lengua/Idioma
dc.contributor.authorLópez Sánchez, Ana-
dc.contributor.authorRamírez García, Vicente-
dc.contributor.authorGarcía-Andrade, Javier-
dc.contributor.authorFlors, Victor-
dc.contributor.authorVera, Pablo-
dc.date.accessioned2012-03-30T10:57:22Z-
dc.date.available2012-03-30T10:57:22Z-
dc.date.issued2011-12-29-
dc.identifier.citationPlos Genetics 7/12:e1002434 (2011)es_ES
dc.identifier.urihttp://hdl.handle.net/10261/47731-
dc.description.abstractRNA–directed DNA methylation (RdDM) is an epigenetic control mechanism driven by small interfering RNAs (siRNAs) that influence gene function. In plants, little is known of the involvement of the RdDM pathway in regulating traits related to immune responses. In a genetic screen designed to reveal factors regulating immunity in Arabidopsis thaliana, we identified NRPD2 as the OVEREXPRESSOR OF CATIONIC PEROXIDASE 1 (OCP1). NRPD2 encodes the second largest subunit of the plantspecific RNA Polymerases IV and V (Pol IV and Pol V), which are crucial for the RdDM pathway. The ocp1 and nrpd2 mutants showed increases in disease susceptibility when confronted with the necrotrophic fungal pathogens Botrytis cinerea and Plectosphaerella cucumerina. Studies were extended to other mutants affected in different steps of the RdDM pathway, such as nrpd1, nrpe1, ago4, drd1, rdr2, and drm1drm2 mutants. Our results indicate that all the mutants studied, with the exception of nrpd1, phenocopy the nrpd2 mutants; and they suggest that, while Pol V complex is required for plant immunity, Pol IV appears dispensable. Moreover, Pol V defective mutants, but not Pol IV mutants, show enhanced disease resistance towards the bacterial pathogen Pseudomonas syringae DC3000. Interestingly, salicylic acid (SA)–mediated defenses effective against PsDC3000 are enhanced in Pol V defective mutants, whereas jasmonic acid (JA)–mediated defenses that protect against fungi are reduced. Chromatin immunoprecipitation analysis revealed that, through differential histone modifications, SA–related defense genes are poised for enhanced activation in Pol V defective mutants and provide clues for understanding the regulation of gene priming during defense. Our results highlight the importance of epigenetic control as an additional layer of complexity in the regulation of plant immunity and point towards multiple components of the RdDM pathway being involved in plant immunity based on genetic evidence, but whether this is a direct or indirect effect on disease-related genes is unclear.es_ES
dc.description.sponsorshipWe acknowledge the financial support of the Spanish MICINN (grants BFU2009-09771 and Consolider-TRANSPLANTA to PV) and Generalitat Valenciana (Prometeo2010/020 to PV). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.es_ES
dc.language.isoenges_ES
dc.publisherPublic Library of Sciencees_ES
dc.relation.isversionofPublisher's version-
dc.rightsopenAccesses_ES
dc.titleThe RNA Silencing Enzyme RNA Polymerase V Is Required for Plant Immunityes_ES
dc.typeartículoes_ES
dc.identifier.doi10.1371/journal.pgen.1002434-
dc.description.peerreviewedPeer reviewedes_ES
dc.identifier.e-issn1553-7390-
dc.identifier.pmid22242006-
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.openairetypeartículo-
item.cerifentitytypePublications-
item.languageiso639-1en-
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
Aparece en las colecciones: (IBMCP) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato
PLOS GENETICS 7_e1002434.pdf750,32 kBAdobe PDFVista previa
Visualizar/Abrir
Show simple item record

CORE Recommender

PubMed Central
Citations

86
checked on 08-mar-2024

SCOPUSTM   
Citations

153
checked on 21-mar-2024

WEB OF SCIENCETM
Citations

140
checked on 23-feb-2024

Page view(s)

401
checked on 27-mar-2024

Download(s)

277
checked on 27-mar-2024

Google ScholarTM

Check

Altmetric

Altmetric


Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.