English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/47708
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

DNA is more, negatively supercoiled in bacterial plasmids than in minichromosomes isolated from budding yeast.

AuthorsMayán-Santos, María Dolores; Martínez-Robles, María Luisa ; Hernandez, Pablo ; Krimer, Dora B. ; Schvartzman, Jorge Bernardo
KeywordsColi seqa protein
Escherichia-coli
Replication forks
topoisomerase-ii
Saccharomyces-cerevisiae
Mitochondrial-DNA
Cell-extracts
Free-energy
Chromatin
Issue Date2007
PublisherJohn Wiley & Sons
CitationElectrophoresis 28:3845-3853(2007)
AbstractA series of circular shuttle vectors were constructed that could replicate and transcribe in the cells of both Escherichia coli and Saccharomyces cerevisiae. 2-D agarose gel electrophoresis run without or in the presence of different concentrations of chloroquine (CHL) revealed that bacterial plasmids were more negatively (-) supercoiled than minichromosomes isolated from budding yeast. Attempts to increase (-) supercoiling in S. cerevisiae or to reduce it in E. coli have deleterious biological consequences. These observations indicate that DNA supercoiling can vary in different species but cells are exquisitely sensitive to sudden changes in supercoiling. In E. coli, the observation that cell growth as well as ColE1 plasmid copy number decrease when DNA relaxes suggests that supercoiling could affect cell viability by regulating the initiation of both transcription and replication.
Description9 páginas; 5 figuras; 1 tabla
Publisher version (URL)http://dx.doi.org/10.1002/elps.200700294
URIhttp://hdl.handle.net/10261/47708
DOI10.1002/elps.200700294
ISSN0173-0835
E-ISSN1522-2683
Appears in Collections:(CIB) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.