Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/47624
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Rodríguez, Enrique | - |
dc.contributor.author | Ruiz-Dueñas, F. J. | - |
dc.contributor.author | Kooistra, Rolf | - |
dc.contributor.author | Ram, Arthur | - |
dc.contributor.author | Martínez, Ángel T. | - |
dc.contributor.author | Martínez, María Jesús | - |
dc.date.issued | 2008 | - |
dc.identifier.citation | Journal of Biotechnology 134(1-2): 9-19(2008) | es_ES |
dc.identifier.issn | 0168-1656 | - |
dc.identifier.uri | 10261/47624 | - |
dc.description | 11 páginas, 4 figuras -- PAGS nros. 9-19 | es_ES |
dc.description.abstract | In this paper we report the cloning and nucleotide sequence analysis of two new laccase genes from the white-rot fungus Pleurotus eryngii, named pel3 and pel4. Comparison of the protein sequences deduced from these genes with laccases previously described in P. eryngii indicates that these genes codify for new laccases in this fungus. We described the expression of pel3 gene in two different Aspergillus niger strains. Both the laccase signal peptide and the glucoamylase preprosequence of A. niger were used to target the secretion of the active enzyme. The highest levels of laccase expression were obtained by combining the last construction with an A. niger strain deficient in extracellular proteases secretion. The characterization of catalytic properties of the recombinant enzyme, together with the setting-up of a heterologous expression system for pel3, will provide the basis to study the biotechnological applications of this enzyme. | es_ES |
dc.description.sponsorship | Contract NMP2-CT-2006-026456 and the Spanish project BIO2003-00621 and S-0505/AMB0100. Financial support received from the “Comunidad de Madrid" | - |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | closedAccess | es_ES |
dc.subject | Basidiomycetes | es_ES |
dc.subject | Phenol oxidases | es_ES |
dc.subject | Copper protein | es_ES |
dc.subject | Protein sequence | es_ES |
dc.subject | Heterologous expression | es_ES |
dc.title | Isolation of two laccase genes from the white-rot fungus Pleurotus eryngii and heterologous expression of the pel3 encoded protein | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1016/j.jbiotec.2007.12.008 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.jbiotec.2007.12.008 | es_ES |
dc.identifier.e-issn | 1873-4863 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
item.languageiso639-1 | en | - |
Aparece en las colecciones: | (CIB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Acceso restringido.pdf | 21,67 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
49
checked on 16-abr-2024
WEB OF SCIENCETM
Citations
46
checked on 27-feb-2024
Page view(s)
212
checked on 18-abr-2024
Download(s)
63
checked on 18-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.