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Título: | p-Coumaric acid decarboxylase from Lactobacillus plantarum: Structural insights into the active site and decarboxylation catalytic mechanism |
Autor: | Rodríguez, Héctor CSIC ORCID; Angulo, Iván CSIC; Rivas, Blanca de las CSIC ORCID; Campillo, Nuria E. CSIC ORCID ; Páez, Juan A. CSIC ORCID; Muñoz, Rosario CSIC ORCID ; Mancheño, Jose M. CSIC ORCID | Palabras clave: | Catalytic mechanisms Crystal structures Decarboxylase Phenolic acids p-coumaric acid |
Fecha de publicación: | 15-may-2010 | Editor: | Wiley-Blackwell | Citación: | Proteins 78(7): 1662–1676 (2010) | Resumen: | p-Coumaric acid decarboxylases (PDCs) catalyze the nonoxidative decarboxylation of hydroxycinnamic acids to generate the corresponding vinyl derivatives. Despite the biotechnological relevance of PDCs in food industry, their catalytic mechanism remains largely unknown. Here, we report insights into the structural basis of catalysis for the homodimeric PDC from Lactobacillus plantarum (LpPDC). The global fold of LpPDC is based on a flattened b-barrel surrounding an internal cavity. Crystallographic and functional analyses of single-point mutants of residues located within this cavity have permitted identifying a potential substratebinding pocket and also to provide structural evidences for rearrangements of surface loops so that they can modulate the accessibility to the active site. Finally, combination of the structural and functional data with in silico results enables us to propose a two-step catalytic mechanism for decarboxylation of p-coumaric acid by PDCs where Glu71 is involved in proton transfer, and Tyr18 and Tyr20 are involved in the proper substrate orientation and in the release of the CO2 product | Versión del editor: | http://dx.doi.org/10.1002/prot.22684 | URI: | http://hdl.handle.net/10261/47581 | DOI: | 10.1002/prot.22684 | ISSN: | 0887-3585 | E-ISSN: | 1097-0134 |
Aparece en las colecciones: | (IQF) Artículos (IFI) Artículos (IQM) Artículos |
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p-Coumaric_acid_decarboxylase_Rodriguez.pdf | 915,15 kB | Adobe PDF | Visualizar/Abrir |
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